银染法与产物杂交法检测端粒扩增产物比较  

Comparison between AgNO_3 staining and hybridization method used to detect telomerase activity in tumor cells

作　　者：雷俊霞[1] 曹孟德[1] 张莹[2] 秦东春[3] 岳宝红 陈宗德[1] 苏堤[1] 刘宏伟[1] 

机构地区：[1]河南医科大学微生物学与免疫学教研室,郑州450052 [2]河南医科大学第一附属医院肿瘤科,郑州450052 [3]河南医科大学第一附属医院检验科,郑州450052

出　　处：《河南医科大学学报》2001年第4期409-410,共2页Journal of Henan Medical University

基　　金：河南省科技攻关资助项目 981170 2 2 7

摘　　要：目的 :选择简便、灵敏地检测端粒酶活性的方法。方法 :以HL 6 0细胞与慢性粒细胞白血病单个核细胞为检测对象 ,在端粒重复序列扩增技术 (telomericrepeatamplificationprotocol,TRAP)基础上 ,采用同步扩增产物杂交法和产物银染法检测其端粒酶活性。结果与结论 :产物杂交法操作简便 ,比产物银染法有更高的灵敏度 ,还可用于半定量。Aim: In order to search for rapid and sensitive method to detect telomerase activity in tumor cells. Methods: AgNO 3 staining and hybridization method based on telomerase repeat amplification protocol(TRAP) were concurrently used to detect telomerase activity in HL 60 strain and mononuclear cells of chronic myeloid leukemia. Results and conclusion: Hybridization method is more sensitive than AgNO 3 staining, moreover,it also can be used to relatively quantify telomerase activity.

关 键 词：端粒酶 TRAP HL-60细胞 慢性粒细胞白血病 银染法 

分 类 号：R733.72[医药卫生—肿瘤]