沙利度胺对小鼠肝癌血管形成的影响  

Effects of thalidomide on liver cancer angiogenesis in mice

作　　者：王喜安[1] 肖正达[1] 金冠球[1] 王洛伟[1] 沈宾鸿[2] 丁艾妮[2] 吕礁[1] 沈敏 

机构地区：[1]解放军第411医院消化内科,上海200081 [2]解放军第411医院病理科 [3]上海市安图医院检验科

出　　处：《第二军医大学学报》2001年第6期561-563,共3页Academic Journal of Second Military Medical University

摘　　要：目的 :研究沙利度胺对小鼠肝癌血管形成的影响 ,并初步探讨其抑瘤机制。方法 :小鼠肝癌 HAC细胞接种于昆明种小鼠前肢腋下皮下 ,建立小鼠肝癌皮下移植瘤模型 ,随机分为阴性对照组 (蒸馏水灌胃 )、阳性对照组 (接种后第 1、3、5天 ip环磷酰胺 10 0 m g/ kg)、沙利度胺 A组和沙利度胺 B组 (分别于接种后第 1、5天灌胃 5 0 m g· kg- 1 · d- 1 沙利度胺 0 .5 m l)。采用Envision System免疫组织化学方法 ,检测肿瘤组织微血管密度 (MVD)、增殖细胞核抗原 (PCNA)和血管内皮细胞生长因子(VEGF)。 结果 :阴性对照组、阳性对照组 MVD分别为 (16 .8± 5 .2 )、(11.5± 2 .8) ,PCNA指数分别为 (5 7.1± 6 .3) %、(6 8.2± 4.1) % ,VEGF阳性率分别为 (11.5± 4.3) %、(5 .4± 3.9) %。沙利度胺 A组、B组 MVD分别为 (2 .9± 1.3)、(10 .5±2 .7) ,PCNA指数分别为 (5 1.6± 3.3) %、(5 5 .7± 18.1) % ,VEGF阳性率分别为 (0 .8± 0 .2 ) %、(2 .2± 1.1) %。沙利度胺组MVD、VEGF阳性率均低于阴性对照组 ,差异显著 (P<0 .0 5 )。阳性对照组、沙利度胺 A组、B组抑瘤率分别为 83.8%、5 0 .1%、41.5 %。结论 :沙利度胺通过抑制小鼠肝癌的血管生成 ,抑制肿瘤生长 ,联合使用其他药物 ,可能在实体瘤的治疗中具有良好的应用?Objective: To study the vascular suppressive effect of thalidomide on mice liver cancer angiogenesis and its mechanism. Methods:The mice liver cancer HAC cell strain was inoculated subcutaneously in general Kun Ming mice to develop experimental animal model. There were 4 groups: negative control group (distilled water perfusion stomach); the positive control group(cyclophosphamide 100 mg/kg, ip 1,3 and 5 d after inoculation); thalidomide A and thalidomide B. In thalidomide A and thalidomide B groups the thildomide (50 mg·kg 1 ·d 1 ) was used 1 and 5 d respectively after inoculation. The MVD,PCNA and VEGF were observed using Envision System immunohistochemistry. Results: In thalidomide A and B groups the MVD were 2.9±1.3 and 10.5±2.7, the positive rate of VEGF were (0.8±0.2)% and (2.2±1.1)% respectively. Both the MVD and the VEGF were significantly lower than those in the negative control group ( P <0.05). The cancer suppressive rate of thalidomide A and B groups were 50.1% and 41.5% respectively. Conclusion: Thalidomide can inhibit the growth of mice HAC liver cancer through its antiangiogenic function, it will have a very strong potential perspective in the future. [

关 键 词：沙利度胺 肝肿瘤 血管形成 小鼠 

分 类 号：R735.7[医药卫生—肿瘤] R543[医药卫生—临床医学]