小鼠肝细胞核凋亡的超微结构观察  

The ultrastructural observation of apoptotic mouse liver cell nuclei

作　　者：吴旻[1] 赵允[1] 翟中和[1] 

机构地区：[1]北京大学生命科学中心,北京100871

出　　处：《电子显微学报》2001年第1期21-26,共6页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目!(3980 0 0 75 ;19890 380 );国家重点基础研究资助项目 !(G19990 5 390 5 )

摘　　要：用胡萝卜细胞胞浆制备的非细胞体系中 ,加入纯化的小鼠肝细胞核并以细胞色素 C诱导细胞核发生凋亡。在透射电子显微镜和扫描电子显微镜下都观察到凋亡的细胞核呈现典型的凋亡形态学特征 ,例如染色质的凝集及趋边化 ,细胞核表面形成空泡化结构并有大量的凋亡小体结构出现。在此基础上对凋亡小体结构在形成及排出核外的过程进行了详细的观察和分析。We herein reported that the apoptosis of mouse liver cell nuclei triggered by cytochrome C in cell free system, which was based on the carrot cell extracts. Observed with transmission electron microscope and scanning electron microscope, the apoptotic nuclei showed the typical morphological feathers of apoptosis: condensation and margination of chromatin, bubbling of nuclear membrane and apoptotic bodies. Then we took a deeper look into the process how the apoptotic bodies were formed and tried to vivify the formation.

关 键 词：细胞色素C 小鼠肝细胞核 非细胞体系 超微结构 透射电镜 扫描电镜 形态学 细胞凋亡 

分 类 号：Q24[生物学—细胞生物学]