番鸭细小病毒强毒株(MDPV-Q)VP_2基因的序列分析  

Sequence Analysis of DNA Encoding VP_2 Structural Protein of Muscovy Duck Parvovirus Q Strain

作　　者：娄华[1] 白挨泉[1] 顾万军[1] 陆英杰[1] 杨德威[2] 刘福安[2] 

机构地区：[1]佛山科技学院动物医学系,广东南海528231 [2]华南农业大学动物医学系,广东广州510642

出　　处：《中国预防兽医学报》2001年第5期324-328,共5页Chinese Journal of Preventive Veterinary Medicine

摘　　要：应用PCR技术扩增了MDPV_Q株的VP2 蛋白基因片段。将扩增后的VP2 基因重组到pMD18_T质粒载体上 ,并对插入片段进行序列测定。将测序结果及由该结果推导的氨基酸序列分别与MDPV、GPV进行了同源性比较及分析。结果表明 ,我国分离的MDPV_Q株其VP2 基因序列与国外分离株具有很高的同源性。而与GPV的同源性较低。The DNA encoding VP 2 structural protein was amplified by PCR and recombined into T vector and sequenced Compare gene and amino acid squence of VP 2 of MDVP_Q with that of GPV and MDPV which were registered in the genbank.It shows that both DNAs encoding VP 2 structural protein of MDPV_Q and reported MDPV have high homology,while the homology with GPV is low.

关 键 词：番鸭细小病毒 鹅细小病毒 基因序列分析 强毒株 

分 类 号：S852.659.2[农业科学—基础兽医学]