用RAPD技术对香菇非对称杂交后代遗传性状的分析  

Analysis of Genetic Characters in Nonsymmetrical Crossing Filial Generations of Lentinula edodes with RAPD Technique

作　　者：谭琦[1] 杨建明[2] 陈明杰[3] 贺冬梅[3] 潘迎捷[3] 黄为一[1] 

机构地区：[1]南京农业大学资源与环境科学学院,南京210095 [2]安徽技术师范学院,凤阳233100 [3]上海市农业科学院食用菌研究所

出　　处：《食用菌学报》2001年第2期10-14,共5页Acta Edulis Fungi

摘　　要：利用RAPD技术 ,对香菇非对称杂交的亲本与后代之间的遗传相关性进行了分析。2 0个随机引物共扩增出 4 7条DNA片段 ,在此基础上分析构建了遗传相关聚类图。结果表明 ,非对称杂交的后代 ,其遗传距离与单核受体较近 ,遗传相似性在 85%左右 ,而与双核供体遗传距离较远 ,遗传相似性在The genetic similarity among the acceptor parent, donor parent and filial generations of nonsym- metrical crossing Lentinula edodes was analyzed with RAPD technigue. 47 DNA fragments were amplified by 20 random primers. And the dendrogram of genetic correlation was constructed on this basis. The result showed that the genetic characters of nonsymmetrical crossing filial generations were similar to that of the mononuclear accepter-parent with a genetic similarity index of about 85%, and that more genetic diversity exsisted between the nonsymmetrical crossing filial generation and the binuclear donor parent with a genetic similarity index of about 61%.

关 键 词：香菇 非对称杂交 RAPD 遗传分析 

分 类 号：S646[农业科学—蔬菜学]