TPD修饰电化学生物传感器测定DNA片段序列  

Sequence Determination of DNA Pieces Using a TPD Modified Electrochemical Biosensor

作　　者：彭图治[1] 程琼[1] 

机构地区：[1]浙江大学化学系西溪校区，杭州310028

出　　处：《化学学报》2001年第7期1125-1129,共5页Acta Chimica Sinica

基　　金：国家自然科学基金，浙江省自然科学基金

摘　　要：合成了一种新的吸附偶联试剂-硫酚乙酸琥珀酰亚胺酯(TPD),将TPD吸附在金的表面,利用其琥珀酰亚胺基与客体氨基的反应,将单链DNA修饰在金电极上作为探针,以电化学方法检测能与之互补DNA样品的碱基序列和含量.杂交后的DNA采用自行合成的双[1-二茂铁氨甲酰丙基-四氢化吡嗪-4-丙基氨甲酰吡啶]并吩嗪(FCZ)作指示剂进行检测,该化合物能够嵌入DNA双螺旋结构的碱基对中,显示出高度电化学活性.该传感器的电流信号与DNA浓度在2×10-3～1×100-7 mol/L范围有线性关系,检测限为8×10-10 mol/L.本方法可用于检测爱滋病和乙肝病毒DNA特征序列的研究.A new modifier N - [6 - (thien - 3 - yl)acetoxy] - pyrrolidine - 2,5 - dione(TPD) and a sensitive electroactive indicator di[1 - (ferrocene - carbamoylpropyl) - tetrahydropyrazine - 4 - (propyl carbamoyl - pyridine) -] phenazine (FCZ) have been synthesized. TPD is adsorbed on gold surface to immobilize ssDNA as probe. FCZ is intercalated in dsDNA and detected by differential pulse voltammetry. The biosensor is based on a chemically modified electrode, on which TPD - ssDNA is self - assembled. It is sensitive to cDNA with the detection limit being 8 x 10(-10) mol/L. The current signal of the biosensor is proportional to the concentration of cDNA in the range of 2 x 10(-8) to 1 x 10(-7) mol/L. This electrochemical biosensor has been used to detect sequence - specific DNAs related to HIV - 1 and HBV virus.

关 键 词：DNA片段 化学修饰电极 示差脉冲伏安法 TPD 电化学生物传感器 序列测定 

分 类 号：Q75[生物学—分子生物学] O657.1[理学—分析化学]