人工模拟加卤酶II.酶学性质  

Haloperoxidase by Imitation Ⅱ.The Enzymological Properties

作　　者：祁超[1] 丁兰[1] 张玉静[2] 刘仔[2] 赵大庆[1] 

机构地区：[1]中国科学院长春应用化学研究所,长春130022 [2]中国人民解放军军需大学生物化学教研室

出　　处：《应用化学》2001年第7期560-562,共3页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金资助项目 (2 970 10 0 5 )

摘　　要：The meso-tetra(α,α,α,α-O-phenylacetylbenzene)porphyrin was used as a complete antigen to elicit monoclonal antibody 1F2 through the immunization and cell fusion techniques. The antibody could combine with Mn porphyrin and showed a haloperoxidase activity. The affinities of the antibody for antigen and Mn porphyrin were 4 98×10 -5 mol/L and 7 63×10 -5 mol/L, respectively. When the antibody-Mn porphyrin complex was used to catalyze the halogenation reaction, the Michaelis constant and reactive rate constant κ cat for H 2O 2 were 5 127 0×10 -3 mol/L and 0 483 4 s -1, respectively. The enzyme exchange number κ cat/K m=94 285 1 mol/(L·s).The meso-tetra(α,α,α,α-O-phenylacetylbenzene)porphyrin was used as a complete antigen to elicit monoclonal antibody 1F2 through the immunization and cell fusion techniques. The antibody could combine with Mn porphyrin and showed a haloperoxidase activity. The affinities of the antibody for antigen and Mn porphyrin were 4 98×10 -5 mol/L and 7 63×10 -5 mol/L, respectively. When the antibody-Mn porphyrin complex was used to catalyze the halogenation reaction, the Michaelis constant and reactive rate constant κ cat for H 2O 2 were 5 127 0×10 -3 mol/L and 0 483 4 s -1, respectively. The enzyme exchange number κ cat/K m=94 285 1 mol/(L·s).

关 键 词：全抗原 加卤酶 meso-四(α α α α-O-苯乙酰苯)卟啉 酶学性质 抗体酶 人工模拟酶 

分 类 号：Q553[生物学—生物化学]