球形幽门螺杆菌致小鼠感染  

THE INFECTION OF COCCOID HELICOBACTER PYLORI TO MICE

作　　者：佘菲菲[1] 林建银[1] 刘君炎[2] 黄琛[1] 苏东辉[3] 吴小茜[1] 陈月秀[1] 

机构地区：[1]福建医科大学病原生物系,福州350004 [2]武汉大学医学院微免教研室 [3]福建医科大学免疫学系

出　　处：《中国人兽共患病杂志》2001年第5期21-23,F002,共4页Chinese Journal of Zoonoses

基　　金：福建省自然基金资助 (基金编号 95A0 0 3)

摘　　要：目的　证明球形幽门螺杆菌体内致病性。方法　用抗生素和水诱导幽门螺杆菌 (Hp)球变 ,螺旋形和球形Hp分别经胃灌注接种BALB/c小鼠 ,实验组小鼠 (各 16只 )接种Hp菌液 0 .4ml/只 ( 10 9/ml) ,接种 4次 ,2w完成 ,对照组 ( 10只 )接种生理盐水。距最后一次接种后 3和 4w分别处死小鼠各半。取胃粘膜组织作细菌学检测 (包括胃粘膜快速尿素酶试验 ,细菌培养鉴定 ,电镜观察 )和组织学检测。结果　螺旋菌、抗生素诱变的球形菌、水中球形Hp这 3个实验组的胃粘膜组织快速尿素酶试验阳性率分别为 93 .8% ( 15 /16)、87.5 % ( 14 /16)、5 0 % ( 8/16) ,由此反映的细菌定居量评分为 1.75、1 13、0 .5 6,细菌培养阳性率分别为 87.5 % ( 14 /16)、75 .0 % ( 12 /16)、68.8% ( 11/16) ,对照组阴性。Hp感染 3w和 4w的实验结果无差异。电镜下可见 3个实验组均有弯曲形、杆状和球形Hp粘附于胃粘膜上。组织学检测发现感染的小鼠胃粘膜呈正常、轻度或重度糜烂、溃疡等不同的表现 ,有炎症细胞浸润。水中球形Hp感染引起的胃粘膜损伤较轻。 结论　球形Hp可致胃炎或溃疡。Aim To testify the pathogenicity of coccoid Helicobacter pylori in vivo.Methods 0.5 ml of culture of H.pylori with spiral and coccoid morphology(10 9/ml),which was induced by antibiotics and water,were inoculated intragastrically in BALB/c mice for four times in two weeks. In 3 and 4 weeks after the last bacteria inoculum, half of the mice were sacrificed respectively.Histology and H.pylori colonization were detected by urease test of gastric mucosa,cultures of H.pylori,and electron microscopy and so on.Results The positive rates of urease test of gastric mucosa ,which was infected by spiral H.pylori,coccoid H.pylori induced by antibiotics,or coccoid H.pylori in water, were 93.8%(15/16),87.5%(14/16),and 50%(8/16) respectively, and the score of the number of H.pylori colonies was 1.75,1.13 and 0.56 respectively. The positive rates of cultures of H.pylori in three experimental groups were 87.5%(14/16),75.5%(12/16),and 68.8%(11/16) respectively. These test results were negtive in controls.The results were no difference from the groups inoculated for 3 and 4 weeks.All three groups showed H.pylori adhering to gastric wall with spiral ,bacillary and coccoid by electron-microscopy. Observing the gastric mucosa infected by H.pylori,there were different degree of erotion, ulcer, and inflammative cells infiltrating. The gastric injury infected by coccoid H.pylori in water was more slighter.Conclusion Coccoid H.pylori may cause gastrititis or gastric ulcer.

关 键 词：球形幽门螺杆菌感染 BALB/C小鼠 致病性 

分 类 号：R57[医药卫生—消化系统] R378.99[医药卫生—内科学]