重组人肝再生增强因子对肝纤维化大鼠基质分解素-1基因表达的影响  

EFFECTS OF RECOMBINANT HUMAN AUGMENTER OF LIVER REGENERATION ON STROMELYSIN-1 GENE EXPRESSION IN RATS WITH LIVER FIBROSIS

作　　者：王爱民[1] 和朝平[1] 杨晓明[2] 李培建[1] 陈惠鹏[2] 贺福初[2] 

机构地区：[1]解放军第252医院,保定071000 [2]军事医学科学院放射医学研究所

出　　处：《解放军医学杂志》2001年第7期500-502,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家 8 63计划资助课题 (编号 863 10 2 0 8 0 5 1)

摘　　要：为了解重组人肝再生增强因子 (hALR)对肝纤维化大鼠基质分解素 1(MMP3 )基因表达的影响 ,建立CCl4 中毒性及人血白蛋白免疫损伤性两种大鼠肝纤维化模型 ,模型完成后予不同剂量hALR治疗 ,在不同的时间点留取大鼠肝组织标本 ,提取总RNA ,用逆转录定量聚合酶链反应 (RT PCR)测定MMP3 的基因表达水平。结果在两种模型中hALR治疗组大鼠肝组织MMP3 的基因表达水平在治疗过程中的不同阶段均明显高于模型组 ;高剂量hALR组大鼠肝组织基质分解素 1的基因表达水平均明显高于低剂量组。提示hALR可增强肝纤维化大鼠MMP3To investigate the influence of recombinant human augmenter of liver regeneration (hALR) on stromelysin 1 gene expression in rats with fibrotic liver. CCl 4 or albumin induced liver fibrosis in rats was established, and different dosages of recombinant human augmenter of liver regeneration were given to rats with liver fibrosis.Liver specimens were obtained at different intervals of treatment , total RNA of liver tissues were isolated and stromelysin 1 gene expression was measured by reverse transcription polymerase chain reaction (RT PCR) . The results showed that in both rat models of experimental liver fibrosis , stromelysin 1 gene expression levels were significantly higher in hALR treated rats than those without the treatment at various intervals. Stromelysin 1 gene expression levels in high dose hALR treatment group were significantly higher than that in low dose hALR treatment group. It suggested that recombinant human augmenter of liver regeneration may enhance stromelysin 1 gene expression in rats with fibrotic liver.

关 键 词：重组人肝再生增强因子 基质分解素-1 基因表达 肝纤维化 大鼠 

分 类 号：R575.2[医药卫生—消化系统]