粪便脱落细胞多基因变异与大肠癌早期诊断的关系  

The relationship between alterations of multiple genes in stools from patients with colorectal carcinoma and the role in early diagnosis

作　　者：盛希忠[1] 申泮远[2] 穆庆岭[2] 王教华 

机构地区：[1]山东省千佛山医院,山东济南250014 [2]山东省立医院 [3]淄博市矿务局双山医院

出　　处：《山东医药》2001年第14期4-6,共3页Shandong Medical Journal

摘　　要：采用ＰＣＲ－ＳＳＣＰ分析技术，检测４０例大肠癌癌组织中抑癌基因ＡＰＣ第１１外显子、ＭＣＣ第１０外显 子和癌基因 Ｋ－ｒａｓ第 １外显子的变异情况，并在 ２０例大肠癌患者粪便中进行三种基因的检测。结果：ＡＰＣ第 １１ 外显子和 ＭＣＣ第１０外显子在 Ａ／Ｂ期变异率明显高于 Ｃ／Ｄ期，Ｋ－ｒａｓ第 １外显子突变率在粘液癌中明显高于腺 癌，且随分化程度降低有降低的趋势；在粪便中均检测到与肿瘤组织中三种基因相应的变异。认为检测粪便脱落 细胞基因变异可用于大肠癌早期诊断，ＡＰＣ／ＭＣ和 Ｋ－ｒａｓ可用于估计大肠癌预后。The changes of exon 11 of APC (adenomatous polyposis coli) gene, exon 10 of MCC(mutated in colorectal carcinoma)gene and exon 1 of K-ras (Kirsten rat sarcoma)gene were separately assayed with PCRSSCP(polymerase chain reaction-single strand conformation polymorphism) technique in 40 samples of colorectal carcinoma and 20 samples from stools of patients with colorectal carcinoma. Results showed the alterative rate of exon 11 of APC and exon 10 of MCC were significantly higher in stage A/B than in stage C/D. The mutation rate of exon 1 of K-ras were significantly higher in mucinous carcinoma than in adenocarcinoma, and the rate had a decreasing tendency with the differentiation decreased. It is thought that detection of APC/MCC and K-ras genes might be used in early diagnosis and prognosis of colorectal carcinoma.

关 键 词：结直肠肿瘤 基因突变 粪便 大肠癌 PCR-SSCP 

分 类 号：R735.34[医药卫生—肿瘤] R730.43[医药卫生—临床医学]