丹参酸乙对转化生长因子β1刺激的大鼠肝星状细胞的观察  

Observation of salvianolic acid B on rat TGFβ_1-stimulated cultured hepatic stellate cells

作　　者：王晓玲[1] 刘平[2] 谭英姿[2] 童普德[1] 蒋文娟[1] 

机构地区：[1]上海中医药大学生物教研室,200032 [2]上海中医药大学肝病研究所,200032

出　　处：《肝脏》2001年第2期96-97,共2页Chinese Hepatology

基　　金：国家杰出青年科学基金资助! (No .3982 51 2 8)

摘　　要：目的　探讨丹参酸乙 (SAB)对转化生长因子 β1(TGFβ1)刺激的大鼠肝星状细胞活化、Ⅰ型胶原及c fos基因表达的影响。方法　原位灌注、消化大鼠肝脏 ,分离肝星状细胞。以不同浓度SAB温育TGFβ1刺激的大鼠肝星状细胞。异硫氰酸胍一步法提取细胞总RNA ,RT PCR法检测目的基因的表达。结果　 1μmol/LSAB和 10 μmol/LSAB可分别抑制Ⅰ型胶原及c fos基因表达 ,但 1μmol/LSAB和 10 μmol/LSAB对SMα actinmRNA均无显著影响。 结论　TGFβ1对体外活化的大鼠肝星状细胞表达SMα actinmRNA无明显影响 ,可促进Ⅰ型胶原和c fosmRNA的表达。SAB可抑制TGFβ1促进细胞Ⅰ型胶原mRNA表达的作用。Objective To investigate the effects of SAB on type I collagen and c fos gene expression, and activation of TGFβ 1 stimulated rat cultured hepatic stellate cell. Methods Hepatic stellate cells were derived from normal rat by perfusion and digestion in situ. Then incubated the TGFβ1 stimulated hepatic stellate cells, with various concentrations of SAB. Total RNA was extracted by acidguanidium single step method. Gene expressions of smooth muscle α actin, type I collagen and c fos were assayed by reverse transcription polymerase chain reaction(RT PCR). Results Both 1 μmol/L SAB and 10 μmol/L SAB suppressed type I collagen and c fos mRNA expression. Neither 1 μmol/L SAB nor 10 μmol/L SAB reduced the smooth muscle α actin mRNA expression. Conclusion TGFβ1 did not affect smooth muscle α actin mRNA expression in activated rat cultured hepatic stellate cells, but increased the type I collagen and c fos mRNA level. SAB could inhibit the type I collagen mRNA expression promoted by TGFβ1.

关 键 词：转化生长因子Β1 丹参酸乙 肝星状细胞 大鼠 肝纤维化 MRNA 

分 类 号：R575.2[医药卫生—消化系统]