用微载体高密度培养CHO工程细胞  

HIGH-DENSITY CULTIVATION OF GENETICALLYENGINEERED CHO CELL LINE WITH MICROCARRIER CULTURE SYSTEM

作　　者：肖成祖[1] 刘凤云[1] 陈昭烈[1] 郭智霞[1] 李琳[1] 高丽华[1] 孔惟惟 于曼[2] 

机构地区：[1]军事医学科学院生物工程研究所 [2]军事医学科学院微生物学流行病学研究所

出　　处：《军事医学科学院院刊》1991年第1期45-50,共6页Bulletin of the Academy of Military Medical Sciences

摘　　要：rβ-13 CHO 工程细胞在 MC-1型微载体上可良好地附着和增殖,当载体浓度为10mg/ml 时,细胞密度可高达4.5×10~6细胞/ml。由于该细胞具有可自动从载体解离,并再附着和增殖于新载体表面的特性,故采用直接加入新鲜载体和培养基的方法,即可方便地扩大生产。这些结果表明用微载体悬浮培养系统生产 CHO 工程细胞产品将是可行的。γβ-13 GHO cell line,which was cotransfected with recombinant plasmid PSVEβ inc- luded HuIFN-β gene and PSV_2-dhfr plasmid,was cultivated with M C-1 microcarrier culture system to high density.The cell density could be enhanced by increasing concentration of microcarrier.When concentration of microcarrier was 10 mg/ml,the cell density could reach 4.5×10~6 cell/ml,These experiments showed that this cell line has the property of being re- leased from microcarrier on its own accord and reattached,proliferated on fresh microcarriers. So we successfully scaled up the cultivation with a simple method,i.e.to add the fresh mic- rocarriers and medium directly into the culture system about 2,4 or 8 times of its original volume. These experiments suggested that microcarrier culture system was a very suitable one for producing large quantity,high quality and low cost products by genetically-engineered CHO cell lines.

关 键 词：微载体 CHO细胞 培养 

分 类 号：R394.8[医药卫生—医学遗传学]