胎肝低分子抑瘤物和维生素A酸对HL-60细胞中c-myc癌基因表达的影响  

COMPARISON OF c-myc GENE EXPRESSION IN HL-60 LEUKEMIA CELLS BY RA AND A LOW MOLECULAR TUMOR SUPPRESSOR OF HUMAN FETAL LIVER ORIGIN

作　　者：刘景梅 邢桂春[1] 薛惠华[1] 吴祖泽[1] 

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《军事医学科学院院刊》1991年第2期84-87,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：国家青年自然科学基金

摘　　要：胎肝中存在着一类低分子抑瘤物,可明显抑制 HL-60细胞的增殖。为探讨其作用机制,本实验对 HL-60细胞经胎肝低分子抑瘤物和维生素 A 酸作用后 c-myc 癌基因的表达水平进行了检测。结果表明,维生素 A 酸可明显抑制 HL-60细胞中 c-myc 基因的表达,而胎肝低分子抑瘤物却无明显作用。HL-60 cell line,derived from a patient with promyelocytic leukemia,can be induced to differentiate in vitro to granulocyte or monocyte/macrophage in the presence of a specific inducing agent such as DMSO,TPA,RA or others.Therefore,HL-60 cells provide an invaluable model system for studying the oncogene structure and its expression under various conditions.In this paper we studied the effect of RA and a newly developed low molecular tumor suppressor isolated from human fetal liver on the expression of c-myc in HL-60 cells. The results indicated that c-myc mRNA level decreased markedly in differentiating HL-60 cells treated with RA.However,under the similar culture system c-myc mRNA level did not change when HL-60 cells were cultured in vitro in the presence of low molecular tumor suppressor of human fetal liver origin.

关 键 词：胎肝 低分子抑瘤物 癌基因 C-MYC 

分 类 号：R730.2[医药卫生—肿瘤]