^(188)Re间接标记单克隆抗体的研究  

Study on indirect method for ^(188) Re labeling monoclonal antibody

作　　者：贾芳[1] 孟昭兴[1] 胡久华[1] 王华[1] 刘伯里[1] 

机构地区：[1]北京师范大学化学系,北京100875

出　　处：《核技术》2001年第8期716-720,共5页Nuclear Techniques

摘　　要：采用先标记后偶联的方法 ,以NHS -MAG3 为双功能连接剂 ,研究了用188Re标记单克隆抗体的各种实验条件 ,得到了最佳标记方法和偶联方法。标记抗体188Re -NHS -MAG3 -IgG体外稳定性良好。完成了188Re -NHS -MAG3 -CL3在小鼠体内的生物分布实验 ,结果表明该配合物在体内稳定性良好。The experimental conditions of 188 Re labeling with antibodies via NHS-MAG 3 as a bifunctional chelating agent were studied by pre-radiolabeling of chelate approach. The conjugation conditions were optimized for the best conjugation performance.The stability of 188 Re-NHS-MAG 3-IgG in vitro and 188 Re-NHS-MAG 3-CL3 in vivo was high.

关 键 词：^188RE 单克隆抗体 NHS-MAG3 双功能连接剂 肿瘤 放射免疫治疗 铼188标记物 放射性药物 间接标记 

分 类 号：R730.55[医药卫生—肿瘤]