用CB微核法预测癌细胞的放射敏感性  

Radiosensitivity of tumor cells predicted by the CB ,micronucleus assay

作　　者：王菊芳[1] 李文建[1] 周光明[1] 何静[1] 李强[1] 党秉荣[1] 颉红梅[1] 卫增泉 高清祥[2] 

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]兰州大学,兰州730000

出　　处：《核技术》2000年第3期187-190,共4页Nuclear Techniques

基　　金：九五国家"攀登计划(B)"项目!950603

摘　　要：用细胞质分裂阻断微核技术（简称ＣＢ微核法）研究了癌细胞的放射敏感性，结果表明；ＣＢ 微核法可用来预测癌细胞对重离子和γ射线的放射敏感性，具有省时且成功率高的特点。细胞 受重离子作用后，每双核细胞的微核与剂量的效应关系适合方程 Y＝ c＋ ｋＤｎ或 Y＝ｃ＋ａＤ＋βＤ２，受γ射线作用后，每双核细胞的微核与剂量的效应关系适合方程 Y＝ｃ＋ ａＤ。在相同剂量下，重离子效应高于ｒ射线效应。Radiosensitivity of tumor cells was studied by the Cytokinesis-Block(CB) micronucleus assay. The results show that radiosensitivity of tumor cells induced by heavy ions and y -rays can be predicted with CB technique, it has the advantages of time saving and higher possibility of success. The dose response for micronuclei per binucleated cells is given by Y= c + kD^n or Y= c + aD+ AD^2 when treated with heavy ions, and given by Y= c + aD in the case ofγ -rays. Under the same dose, effectiveness of heavy ions is stronger than that of γ -rays.

关 键 词：CB微核法 癌细胞 重离子 Γ射线 放射敏感性 预测 癌症 放射治疗 

分 类 号：R730.55[医药卫生—肿瘤]