L6565白血病克隆细胞培养上清液的病毒特性和致病性  

Virological Characteristics and Pathogenicity of Culture Supernatant of L6565 Leukemia Clonal Cells

作　　者：胡安群[1] 姜延芳[1] 吴春根[1] 郑葆芬[1] 

机构地区：[1]复旦大学基础医学院分子生物学实验室

出　　处：《复旦学报（医学版）》2001年第4期296-298,302,共4页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金(395 70 16 5 )资助

摘　　要：目的　探讨小鼠L6 5 6 5克隆细胞株培养上清液中病毒的特性及其致病性。方法　从XC试验、逆转录酶活性、NB嗜性等方面了解培养上清液中病毒的特性 ;将培养上清液和超离心浓缩液分别皮下注射SSB系新生小鼠 ,观察其致白血病活性。结果　L6 5 6 5克隆细胞培养上清液的XC试验呈阳性 ,并具有逆转录酶活性和NB嗜性。培养上清液能诱发T淋巴细胞白血病 /淋巴瘤 (TL)和B淋巴细胞白血病 /淋巴瘤 (BL) ,发病率为 5 0 % (5只 / 10只 ) ;超离心浓缩液可诱发TL、BL和粒细胞白血病 (GL) ,发病率为 10 0 % (10只 / 10只 )。结论　小鼠L6 5 6 5白血病克隆细胞株培养上清液中存在具NB嗜性的、可诱发SSB系新生乳鼠TL。Purpose: To study the virological and pathogenic characteristics in culture supernatant of murine L6565 clonal cells. Methods: We studied the virological and biological characteristics of culture supernatant of murine L6565 clonal cells by XC assay, reverse transcriptase activity and NB tropic. The pathogenic characteristics of culture supernatant of murine L6565 clonal cells which was inoculated Subcutaneously into neonatal SSB strain mice. Results: The culture supernatant of murine L6565 clonal cells showed positive with XC assay, reverstranscriptase activity and NB tropic test. This supernatant induced T lymphocytic leukemia/lymphoma (TL) and B lymphocytic leukemia/lymphoma (BL); the ultracentrifugal concentrated supernatant induced TL, BL and Granulocytic leukemia (GL). Conclusions: There are murine leukemia viruses(MuLV) with NB tropic in the culture supernatant of murine L6565 clonal cells, which can induce TL and BL and GL on neonatal SSB strain mice.

关 键 词：白血病病毒 白血病 基因重排 XC实验 NB检测 

分 类 号：R733.7[医药卫生—肿瘤] Q78[医药卫生—临床医学]