利用突变技术研究苏云金杆菌杀虫晶体蛋白的进展  

Advances in Studies on Bacillus thuringiensis Insecticide Crystal Protein by Mutagenesis

作　　者：汤慕瑾[1] 余健秀[1] 李建华[1] 庞义[1] 

机构地区：[1]中山大学生物防治国家重点实验室昆虫研究所,广州510275

出　　处：《生物工程进展》2001年第4期54-56,42,共4页Progress in Biotechnology

基　　金：国家"8 6 3"项目(86 3-10 1-0 3-0 1-0 1);国家自然科学基金(3990 0 0 98)

摘　　要：本文综述了近年来利用突变技术研究苏云金杆菌 (Bacillusthuringiensis,Bt)杀虫晶体蛋白(Insecticidalcrystalproteins ,ICP)杀虫作用机制所取得的进展。其中结构域Ⅰ参与不可逆结合及影响离子通道的形成 ;结构域Ⅱ参与与受体的结合 ,包括可逆结合和不可逆结合 ;结构域Ⅲ保持三维结构稳定 ,同时可能参与结合受体的过程 ,插膜以及离子通道调节。The current research on the mechanism of the action of Bacillus thuringiensis (Bt) insecticidal crystal proteins by mutagenesis is reviewed.Domain Ⅰ is involved in insertion into the membrane and affects ion channel function.Domain Ⅱ is involved in receptor binding,including the reversible binding and irreversible binding.Domain Ⅲ can maintain the structure stability,and is maybe involved in ion channel function,receptor binding and insertion into the membrane.It is the captivating way of Bt improvement to alter the primary structure of Bt insecticidal crystal proteins by mutagenesis.

关 键 词：苏云金杆菌 杀虫晶体蛋白 结构域 点突变 

分 类 号：S482.39[农业科学—农药学] Q939.124[农业科学—植物保护]