9-顺-维甲酸对人肺鳞癌细胞分化和凋亡的影响  

EFFECT OF 9-CIS-RETINOIC ACID ON DIFFERENTIATION AND APOPTOSIS OF HUMAN LUNG CARCINOMA CELL LINES

作　　者：于雪艳[1] 田克立[1] 张世国[2] 丁磊[1] 胡国强[3] 

机构地区：[1]山东大学医学院生物化学与分子生物学教研室 [2]济南军区总医院检验科 [3]山东大学齐鲁医院胸外科

出　　处：《山东医科大学学报》2001年第4期289-291,共3页Acta Academiae Medicinae Shandong

基　　金：国家自然科学基金资助课题 (3 9770 73 0 )

摘　　要：目的 :研究 9 顺 维甲酸 (9 cis RA)对人肺鳞癌L78细胞、A2细胞分化和凋亡的影响。方法 :以 1和 5 μmol/L 9 cis RA处理肿瘤细胞株 ,分别于 0、12、2 4、36、4 8、72h检测对照组和用药组细胞数及细胞HE染色后的形态变化 ,应用流式细胞术分析细胞周期分布、Bcl 2表达和凋亡细胞数。结果 :9 cis RA可抑制肺鳞癌细胞生长 ;用药后细胞分化征明显 ,可见凋亡细胞。流式细胞计数显示 ,用药组细胞G0 /G1百分率明显升高 ,S期细胞百分率下降 ,细胞凋亡率增加 ,Bcl 2的表达下降。结论 :9 cis RA能抑制人肺鳞癌L78细胞、A2细胞增殖 ,诱导细胞分化和凋亡。Objective:To investigate the effect of 9 cis RA on the differentiation and apoptosis of human lung squamous carcinoma cell lines(L78 and A2).Methods:Cell number and morphology were observed at 0,12,24,36,48,72h respectively after the treatment to the two study groups,the effect of 9 cis RA on the cell cycle and expression of Bcl 2 in L78 and A2 was also examined.Results:9 cis RA significantly decreased the number of L78 and A2 ( P<0.05 ,24h; P< 0.01,36h).Studies implicated G1 phage of the cell cycle was arrested,the percentage of G0/G1 phage increased( P< 0.01,22h),and the percentage of S phage cells decreased( P< 0.01,24h).Cell cycle arrest was associated with increased apoptosis and decreased expression of Bcl 2 protein.Conclusion:9 cis RA potently inhibited the growth of human squamous cancer cells in vitro,induced the differentiation and apoptosis of the cells.

关 键 词：9-顺维甲酸 人肺鳞癌细胞 肺肿瘤 细胞分化 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤] R977.21[医药卫生—临床医学]