血清饥饿和表皮生长因子上调人肝癌细胞中血管内皮生长因子mRNA表达的研究  

UP-REGULATION OF VASCULAR ENDOTHELIAL GROWTH FACTOR mRNA EXPRESSION BY EGF AND SERUM-STARVATION IN HUMAN HEPATOMA CELLS

作　　者：高艳景[1] 袁孟彪[1] 辛华[2] 卢勇[3] 王茜[2] 邵洪莲[2] 

机构地区：[1]山东大学齐鲁医院消化内科 [2]山东大学医学院生物学教研室 [3]山东大学医学院遗传学教研室

出　　处：《山东医科大学学报》2001年第4期304-306,共3页Acta Academiae Medicinae Shandong

摘　　要：目的 :探讨血清饥饿及表皮生长因子 (EGF)对人肝癌细胞血管内皮生长因子 (VEGF)基因表达的调节。方法 :以次黄嘌呤磷酸核糖转移酶 (HPRT)为内标 ,将VEGF与HPRT比值作为VEGF表达水平的参数 ,对VEGFPCR产物相对定量 ,分析血清饥饿和 5 ,2 5ng/ml浓度的EGF在 6h时对人肺癌细胞VEGFmRNA表达水平的影响。结果 :对照组、血清饥饿组、5ng/ml、2 5ng/mlEGF刺激组VEGFmRNA表达的相对量分别为 (2 4 73± 3 19) %、(5 2 73± 9 5 8) %、(76 3 0± 10 78) %和 (114 87± 13 5 5 ) % ,并且EGF组VEGFmRNA的表达水平比血清饥饿组高 (P <0 0 5 )、2 5ng/mlEGF刺激组明显高于 5ng/mlEGF刺激组 (P <0 0 5 )。结论 :血清饥饿和EGF能刺激人肝癌细胞VEGF基因的表达 ,提示 :在人肝癌细胞生长过程中 ,实质性肝癌细胞的生长可能是呈现出一种“周期性爆炸性生长”的方式。Objective:To investigate the VEGF gene expression in human HCC cells by serum starvation and EGF.Methods:The HCC cells were treated with the serum withdrawal culture medium and the concentration of EGF of 5,25ng/ml in serum withdrawal DMEM medium respectively for six huurs.With HRPT as internal standard control,the ratio of VEGF and HPRT as the relative VEGF gene expression level in each group was evaluated by reverse transcriptase polymerase chain reaction.Results:The relative VEGF mRNA levels of the control group,serum withdrawal group,5ng/ml EGF stimulated group and 25ng/ml EGF stimulated group were (24.73±3.18)%,(52.73±9.58)%,(76.30±10.78)%,and (114.87±13.55)% respectively.The relative VEGF mRNA level of the EGF stimulated groups was higher than that of serum withdrawal group ( P<0.05), while the relative VEGF mRNA level of the 25ng/ml EGF stimulated group more higher than that of 5ng/ml EGF stimulated group (P<0.05). Conclusion:Serum starvation and EGF can stimulate the expression of VEGF gene in human HCC cells.According to our present results and other reports,we suggested that the growth of solid HCC cells may show an ″periodic explosive growth pattern.″

关 键 词：血清饥饿 表皮生长因子 尿抑胃素 内此生长因子 肝肿瘤 逆转录-聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤]