丙酮酸高产菌株的选育及中试研究  

Breeding of pyruvate overproducing strain and pilot-scale fermentative production

作　　者：李寅[1] 陈坚[1] 陈燕[1] 黄敏[1] 芮新生 潘春 

机构地区：[1]江南大学工业生物技术教育部重点实验室,无锡214036 [2]常州常茂生物化学工程有限公司,常州213016

出　　处：《工业微生物》2001年第2期10-13,共4页Industrial Microbiology

基　　金：江苏省"九五"工业重大科技攻关项目 (BG980 15 -3)

摘　　要：对T .glabrataWSH -IP12进行EMS诱变 ,挑选以NH4 Cl为唯一氮源的平板上透明圈较大的菌株 ,经初筛和复筛后 ,发现T .glabrataWSH -IP30 3生产丙酮酸的能力强且稳定。以NH4 Cl为唯一氮源摇瓶培养 4 8h ,其丙酮酸产量 (35 .1g/L)比出发菌株 (2 1.4 g/L)提高了 64%。采用该菌株在 30 0L罐上进行了 4批发酵试验 ,丙酮酸产量最高可达 5 8.4g/L ,对葡萄糖产率0 .5 62 g/ g。Mutants which exhibited large transparent rings in the plate with NH 4Cl as a sole nitrogen source, were obtained after ethyl methane sulfonate (EMS) treatment of T. glabrata WSH-IP12. Among them, a mutant WSH-IP303, not only produced pyruvate to a high level with NH 4Cl as a sole nitrogen source, but also showed strong genetic stability during the generation. In a defined medium containing glucose, NH 4Cl, salts and vitamins, pyruvate production by T. glabrata WSH-IP303 reached 35.1 g/L by 48 h flask culture, which was 64% higher than the parent strain. Pyruvate concentration and yield reached 58.4 g/L and 0.562 g/g, respectively, in a 300 litre pilot-scale fermentor.

关 键 词：丙酮酸 光滑球拟酵母 发酵 中试 高产菌株 选育 

分 类 号：TQ920[轻工技术与工程—发酵工程]