原发性非小细胞肺癌中p16/MTS1基因外显子纯合性缺失的研究及临床意义  

Study on the homozygous deletion of multiple tumor suppressor gene in non-small cell lung cancer and its clinical significance

作　　者：费广鹤[1] 桂淑玉[1] 庞玉瑛[1] 张安成[1] 刘虎[2] 周青[2] 汪渊[2] 

机构地区：[1]安徽医科大学第一附属医院呼吸内科,合肥230022 [2]安徽医科大学分子实验室,合肥230032

出　　处：《安徽医科大学学报》2001年第3期199-202,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教委自然科学基金资助 (编号 96JL 0 77)

摘　　要：目的　检测原发性非小细胞肺癌 (Non smallCellLungCancer,NSCLC)手术标本 p16基因 (MultipleTumorSuppressorGene,MTS1)第一、二外显子纯合性缺失并探讨其与肿瘤细胞分化程度、组织类型、转移和预后的关系。方法　收集我院 1999年 1月～ 12月原发性支气管肺癌且手术病理证实为非小细胞肺癌 32例。提取DNA ,建立PCR方法分析 p16基因第一、二外显子纯合性缺失 ,用SouthernBlotting方法对p16第二外显子的PCR产物进行鉴定。 结果　 32例NSCLC患者中 9例发生 p16基因第二外显子的缺失 ,缺失率为 2 8% (9/ 32 ) ,p16第一外显子未发现缺失。结论　在原发性非小细胞肺癌中 ,p16基因第二外显子的纯合性缺失是 p16基因失活的一个重要方式 ,且影响肺癌细胞其他生物学行为。Objective The homozygous deletion of MTS1 gene (p16)of the patients with non small cell lung cancer (NSCLC) was investigated and combined their clinical information to explore the association with the histological type of differentiation and prognosis. Methods 32 surgical samples of Chinese patients with NSCLC were diagnosed by pathology. DNA was extracted from these tissues and the homozygous deletion of p16 gene including exon 1 and 2 were detected with polymerase chain reaction (PCR). The PCR products of p16 exon 2 were analyzed by Southern blotting. Results The homozygous deletion of p16 exon 2 were found in 9 cases of 32 cases. The homozygous deletion rate of p16 exon 2 was 28%. No homozygous deletion of p16 exon 1 were detected in these samples. Conclusion In these NSCLC patients the homozygous deletion of p16 exon 2 may be a major way of the inactivation of p16 gene and affected biological behaviour of lung cancer.

关 键 词：原发性非小细胞肺肿瘤 基因外显子 P16/MTS1 聚合酶链反应 基因纯合性缺失 

分 类 号：R734.2[医药卫生—肿瘤]