113例白血病免疫分型特点分析  

Analysis of immunological classification of 113 cases of leukemia

作　　者：戴海明[1] 翟志敏[1] 何晓东[1] 吴竞生 孙自敏[1] 

机构地区：[1]安徽省立医院中心实验室,合肥230001

出　　处：《安徽医科大学学报》2001年第3期207-210,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金资助课题 (编号 9842 2 12 8)

摘　　要：目的　对 113例白血病免疫分型结果进行回顾性分析 ,总结不同人群免疫分型的特点 ,并将之与FAB初诊结果进行比较。方法　细胞膜抗原采用全血单克隆抗体直接双标记法 ,胞浆内抗原CyMPO ,CyCD3,CyCD2 2的检测在对细胞膜进行通透性处理后 ,采用单色荧光标记 ,流式细胞仪检测。分型根据抗体积分系统。结果　① 16例儿童急性白血病中 ,未分化型占 6 3% ,AML占 43 7% ,B ALL占 31 3% ,T ALL占 12 5 % ,BAL占 6 3% ,90例成人急性白血病中 ,未分化型占 1 1% ,AML占 6 5 6 % ,B ALL占 17 8% ,T ALL占 3% 3% ,BAL占 11 1% ,NK AL占 1 1% ,儿童AML/ALL为 1∶1,明显低于成人AML/ALL的 3 12∶1。②7例慢粒急变患者全部CD34阳性。③发现急性NK细胞白血病 1例 ,CD3-CD38+ 92 % ,CD3-CD5 6 + 88%。结论　白血病免疫分型对于白血病的诊断、治疗和预后判断均有很大帮助 ,其在临床诊断中的推广将有助于病人的诊断和治疗。Objective To analyze immunological classification of 113 cases of leukemia, make a conclusion of immunological characteristics of different groups. Methods Two or one color monoclonal antibodies was used to label whole blood directly, flow cytometry were used to detect, the criteria for classification was according to antibody integral system. Results ① In 16 child patients, the incidence of NULL, AML, ALL, BAL was 6 3%, 43 7%, 43 7%, 6 3%, respectively, In 90 adult patients, the incidence of NULL, AML, ALL, BAL, NK AL was 1 1%, 65 6%, 22 2%, 11 1%, 1 1%, respectively, the rate of AML/ALL of child patients was obviously less than that of the adult patients. ② All of 7 patients with CML blast crisis were CD34 positive. ③A patient with acute NK cell leukemia was found, its positive rate of CD3 -CD38 + and CD3 -CD56 + was 92% and 88%, respectively. Conclusion Immunological classification of acute leukemia is beneficial to its diagnosis, treatment and prognosis judgement.

关 键 词：白血病 免疫学 免疫表型分型 抗原 CD34 

分 类 号：R733.7[医药卫生—肿瘤]