ISSR标记在小麦指纹图谱分析中的应用研究初探  

Preliminary Study of ISSR Marker Technique on Wheat Fingerprinting

作　　者：王心宇[1] 陈佩度[1] 亓增军[1] 张政值[1] 马正强[1] 

机构地区：[1]南京农业大学细胞遗传研究所,江苏南京210095

出　　处：《农业生物技术学报》2001年第3期261-263,W002,共4页Journal of Agricultural Biotechnology

摘　　要：对ＩＳＳＲ标记技术在构建多态水平很低的小麦种内指纹图谱方面做了初步研究。发现（ＡＧ）ｎ、（ＡＣ）ｎ、（ＴＧ）ｎ等二核苷酸重复引物类型在小麦中多态水平最高，能将亲缘关系很近的品种或品系区分开。结果表明：与其它常规分子标记技术如ＲＦＬＰ、ＲＡＰＤ等相比，ＩＳＳＲ标记具有简便、快捷、成本低、多态水平高等特点，是一种值得进一步研究的分子标记技术。The potential of DNA fingerprinting using ISSR marker within intraspecies of wheat lines which had been proved to have lower polymorphism was preliminarily studied.It was found that primers with dinucleotide motifs such as(AG)n?(AC)n?(TG)n could produce the most polymorphic bands on wheat.The results indicated,ISSR marker system,compared to other commonly used marker technique such as RFLP?RAPD etc.,was shown to be an effective marker type characterized by its simplicity,rapidness,cheap cost and high polymorphisms and be suitable for use in wheat fingerprinting analysis.

关 键 词：小麦 DNA指纹图谱 ISSR标记 

分 类 号：S512.1[农业科学—作物学]