鸡生长激素基因的PCR-RFLPs分析  

PCR-RFLPs Analysis of Chicken Growth Hormone Gene

作　　者：聂庆华[1] 张细权[1] 杨关福[1] 梁志清[2] 

机构地区：[1]华南农业大学动物科学系,广东广州510642 [2]香港大学动物学系

出　　处：《农业生物技术学报》2001年第3期282-285,共4页Journal of Agricultural Biotechnology

基　　金：广东省自然科学基金(970012);国家重点基础研究发展规划(G2000016102)资助

摘　　要：本实验以粤黄鸡矮脚黄系等不同蛋鸡、肉鸡品种（系）作为实验材料，研究生长激素（ｃＧＨ）基因的多态性，由此计算鸡不同群体的基因型频率和基因频率，并用ＤＴ软件的类平均法（ＵＰＧＭＡ）进行聚类分析。发现ｃＧＨ内含子１有６种基因型，受３个等位基因控制；内含子４有７种基因型，受４个等位基因控制，丝毛鸡的两个个体的ＰＣＲ产物则出现双带现象。还发现优质肉鸡、速生型肉鸡内含子１和４的基因型频率和基因频率比较接近，而蛋鸡与它们的差别比较明显。聚类分析有类似的结果。Different chicken breeds(lines)such as Yuehuang DH were used and restricted fragment length polymorphisms(RFLPs)in Intron 1 and Intron 4 of cGH gene were investigated in the present study.The allele frequencies of different populations were calculated and clustering analysis was conducted based on allele frequencies.The results showed that there were 6 kinds of genotypes in Intron 1,which were determined by 3 alleles.Seven genotypes determined by 4 alleles were found in intron 4.Two bands were found in PCR product of two samples of Taihe Silkies.The result also showed that quality chickens and broiler chickens had almost the same genotype frequencies and allele frequencies,but layer chickens had different ones.The clustering analysis showed similar results.

关 键 词：鸡 生长激素基因 内含子 多态性 基因频率 地方品种 中国 

分 类 号：S831.2[农业科学—畜牧学]