黄瓜花叶病毒香蕉株系(CMV-Xb)RNA3 cDNA的克隆和序列分析  

Construction of cDNA Clone and Nucleotde Sequence of RNA3 of Cucumber Mosaic Virus Isolate Xb from Banana

作　　者：王海河[1] 谢联辉[1] 林奇英[1] 

机构地区：[1]福建农业大学植物病毒研究所

出　　处：《中国病毒学》2001年第3期252-256,共5页Virologica Sinica

摘　　要：通过RT PCR方法 ,设计两对引物 ,克隆了黄瓜花叶病毒香蕉株系 (CMV Xb)RNA3 ,并进行了核苷酸和蛋白质水平上的分析。结果表明Xb株系RNA3全长 2 2 0 5nt,具有两个蛋白编码阅读框架 (ORF) ,其中 5’端 (97～ 936nt)编码一个 2 79aa的 3a蛋白 ;3’端 (12 2 5～ 1871nt)编码一个 2 18aa的CP蛋白。 5’非编码区域为 96nt;基因间隔区 (IR)长2 88nt;3’NR含有 32 4nt。通过与亚组Ⅱ其它株系RNA3核苷酸和所编码产物推导的氨基酸序列分析发现 ,亚组Ⅱ株系无论在编码区还是非编码区的核苷酸同源性都相对较高 ;亚组Ⅱ株系在进化过程中具有连续性。The nucleotide sequence of RNA3 of a cucumber mosaic virus strain from banana of subgroup Ⅱ(CMV-Xb) was cloned and analyzed at nucleotide and protein level after RT-PCR amplificaion with two pairs of primers.The results showed that CMV-Xb is composed of 2205 nucleotides(nt) with two open reading frames.The one near 5'termini(97～936nt) encodes a 3a protein with 279 amino acids(aa),the other near 3'termini (1225～1871nt)codes for coat protein with 218aa,The 5'non-coding region(NR),intercistron region(IR) and 3'NR are 96nt,288nt and 324nt, respectively.Comparison at the nucleotide and putative amino acid level with other strains in subgroup Ⅱ showed that the nucleotide sequence of Xb is very conservative in the encoding regions as well as non-coding regions.The evolution of RNA3s of subgroup Ⅱ members of CMV is consecutive.

关 键 词：黄瓜花叶病毒香蕉株系 RNA3 全长CDNA克隆 序列分析 

分 类 号：S432.4[农业科学—植物病理学]