利用完整细胞不对称合成R-苯乙醇胺的研究  

Studies on Asymmetric Synthesis of R-phenylethanolamine by Whole Cells of Arachnia sp. P163

作　　者：王佳亮[1] 王建军[1] 杨柳[1] 赵国刚[1] 吴襟[1] 孙万儒[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《生物工程学报》2001年第4期467-470,共4页Chinese Journal of Biotechnology

基　　金：国家自然科学基金资助的重大项目! (3 9670 846)&&

摘　　要：Effects of various factors on asymmetric synthesis of R-phenylaminoethanol from aminoacetophenone by the whole cells of \%Arachnia sp.\% P163 producing alcohol dehydrogenase for phenylethanol amine was investigated. It found that, although the reduction was inhibited by the substrate and the product, but it has the very high stereoselectivity. The reduction was normaly carried out with 2% glucose for reproduction of coenzyme in the reaction system without oxygen. The conversion yield and ee value of the product achieved 65% and 100%, respectively.Effects of various factors on asymmetric synthesis of R-phenylaminoethanol from aminoacetophenone by the whole cells of \%Arachnia sp.\% P163 producing alcohol dehydrogenase for phenylethanol amine was investigated. It found that, although the reduction was inhibited by the substrate and the product, but it has the very high stereoselectivity. The reduction was normaly carried out with 2% glucose for reproduction of coenzyme in the reaction system without oxygen. The conversion yield and ee value of the product achieved 65% and 100%, respectively.

关 键 词：氨基苯乙酮 R-苯乙醇胺 醇脱氢酶 手性 不对称合成 药物 邻羟基胺类 蛛菌细胞 

分 类 号：TQ464[化学工程—制药化工]