补料培养提高真养产碱杆菌产3-羟基丁酸和3-羟基戊酸共聚物生产强度的研究  

High-level Production of Poly (3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Feb-batch Culture of Alcaligenes eutrophus

作　　者：蔡以滨[1] 刘墨青[1] 易祖华[1] 陈琦[1] 翁维琦[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《生物工程学报》2001年第5期510-514,共5页Chinese Journal of Biotechnology

基　　金：国家九五攻关项目(96 C0 3 0 3 0 2 )&&

摘　　要：对Alcaligeneseutrophus进行高密度培养 ,研究表明在发酵过程中进行有效控制 ,可以较大幅度地提高 3 羟基丁酸和 3 羟基戊酸共聚物 [P(3HB co 3HV) ]的生产强度。实验中选择使用限氮的方法积累P(3HB co 3HV) ,分别采用丙酸和戊酸为 3HV前体 ,对摇瓶种子生长状态 ,停氮时机对菌体生产P(3HB co 3HV)的影响以及补酸 (3HV前体 )策略进行了研究 ,在 6 6L罐中 ,以葡萄糖为碳源 ,以丙酸为 3HV前体培养 5 0h ,细胞干重 ,PHA产量 ,PHA含量分别达到 149 9g L ,12 4 9g L ,83 3% (其中 3HV组分占PHA的 12 4mol% ) ,生产强度达到 2 5 0 (g·h- 1 ·L- 1 ) ;以戊酸为3HV前体培养 45h ,细胞干重 ,PHA产量 ,PHA含量分别达到 16 0 2g L、119 0g L、74 2 % (其中 3HV组分占PHA的17 7mol% ) ,生产强度达到 2 6 4(g·h- 1 ·L- 1 )Fermentation strategies for production P (3HB co 3HV) from glucose and propionic (or valeric) acid by Alcaligenes eutrophus were studied.During the culture,we controlled pH of the broth by feeding precusors of 3HV\| propionic or valeric acid after Ammonia feeding stopped.When propionic acid were used as the precusor,for 50 hours,we obtained a cell dry weight,a P(3HB co 3HV) concentration,a P(3HB co 3HV) content and a 3HV fraction of 149 9g/L,1249g/L,833% and 124mol%,respectively,with a PHA productivity of 250 gh -1 L -1 .When valeric acid were used as the precusor,for 45 hours,we obtained a cell dry weight,a P(3HB co 3HV) concentration,a P(3HB\|co\|3HV)content and a 3HV fraction of 160.2g/L,119.0g/L,74.2% and 17.7mol%,respectively,with a PHA productivity of 2.64gh -1 L -1 .Prior to this study,it hasn′t been reported to obtain such high level productivity and 3HV fraction at the same time by Alcaligenes eutrophus.

关 键 词：真养产碱杆菌 3-羟基丁酸 3-羟基戊酸共聚物 生产强度 补料培养 生物降解性 

分 类 号：TQ320[化学工程—合成树脂塑料工业]