葛根素对星形胶质细胞肿胀及大鼠脑水肿的对抗作用  

Pretreatment with Puerarin Against Astrocyte Swelling in Culture and Brain Edema in Rats

作　　者：袁芳[1] 王忠诚[1] 王天佑[1] 徐立新[1] 张亚卓[1] 

机构地区：[1]北京市神经外科研究所

出　　处：《首都医科大学学报》2001年第3期206-208,共3页Journal of Capital Medical University

基　　金：国家自然科学基金 ( 30 0 70 2 34 );北京市科技新星计划 ( 95 38130 0 0 );北京市自然科学基金 ( 70 0 2 0 11)

摘　　要：为观察葛根素是否有对抗星形胶质细胞肿胀及脑水肿的作用 ,体外实验用培养的星形胶质细胞进行 ,整体实验用Wistar雄性大鼠tACPD(5 0 0nmol,2 μL)尾状核内微量注射的方法 ,分别测定星形胶质细胞水含量、脑组织水含量和Na+ 、K+ 、Ca2 + 含量 ,部分脑组织进行电镜观察。结果显示葛根素可以阻断tACPD引起的星形胶质细胞水含量增加 ,减轻由tACPD尾状核内注射引起的脑组织水含量和Na+ 含量增加 ,电镜检查证实葛根素可以减轻tACPD引起的星形胶质细胞肿胀及足突肿胀对毛细血管的挤压。提示葛根素可以对抗星形胶质细胞肿胀及脑水肿。The effects of puerarin (pue), an effective component of a Chinese herb, against astrocyte swelling in cultures and brain edema intact rats were studied. Astrocyte swelling in culture was induced with trans 1 aminocyclopentane 1, 3 dicarboxylic acid (tACPD), an agonist of metabotropic glutamate receptors. tACPD (1 mmol/L) alone or with pue (1 mmol/L) were added in culture at 37 ℃ for 1 h, then the intracellular water space (IWS) was measured by 3H 3 O methyl D glucose. For in vivo study, brain edema was induced with tACPD (500 nmol) in 2 μL injected stereotactically into right caudatum of the rat. Pue (50 mg/kg) was administered intraperitoneally on the 1st and 2nd day, and intravenously on the 3rd day, then followed by intracaudatal injection of tACPD. Six h after tACPD injection brain water content and Na +, K +, Ca 2+ contents were determined. Histological study was performed with electron microscope (EM). It was showed that pue blocked the increase of IWS induced by tACPD in astrocyte culture and reduced the increases of brain water and Na + contents induced by tACPD in vivo. Furthermore, astrocyte swelling and the constriction of capillary lumen induced by the pressure of swelling endfeet of astrocytes, which were observed in tACPD treated rats under EM, were attenuated by pue treatment. The results indicats that pretreatment with pue can prevent astrocyte swelling and brain edema induced by tACPD, which may be beneficial to brain microcirculation during hypoxia and ischemia.

关 键 词：星形胶质细胞肿胀 脑水肿 葛根素 实验研究 

分 类 号：R742.7[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]