PCNA与nm23在膀胱移行细胞癌中的表达及意义  

Expression of PCNA and nm23 in transitional cell carcinoma of bladder

作　　者：温凯胜[1] 张亚正[1] 贾俊英[1] 闫骏[1] 黄勇[1] 朱小军[1] 

机构地区：[1]内蒙古医学院附属医院泌尿外科,内科,内蒙古呼和浩特010050

出　　处：《内蒙古医学杂志》2001年第4期293-297,共5页Inner Mongolia Medical Journal

摘　　要：目的 :研究增殖细胞核抗原 (PCNA)与转移抑制基因 (nm2 3)在膀胱移行细胞癌中的表达 ,发现其与膀胱移行细胞癌生物学行为的关系及其临床和病理意义。方法 :采用免疫组化S -P法 ,测定PCNA、nm2 3在 78例膀胱移行细胞癌中的表达。用阳性水平指数 (PLT)作为表示PCNA、nm2 3基因蛋白表达水平指标。阳性水平指数(PLI) =阳性细胞百分率P/A(% )×平均光密度 (AOD)。结果 :8例正常膀胱黏膜细胞核均有PCNA阳性表达。与肿瘤相比 ,差异具有显著性 (P <0 0 1 )。 8例正常膀胱黏膜均有nm2 3表达 ,与肿瘤相比 ,差异具有显著性 (P <0 0 1 )。PCNA在膀胱移行细胞癌中表达与分期呈正相关 (P <0 0 1 ) ,与分级呈正相关关系 (P <0 0 1 ) ,与肿瘤大小呈正相关 (P <0 0 1 )。nm2 3在膀胱移行细胞癌中表达与分期、分级、大小不相关。二者在膀胱移行细胞癌中的表达均与复发与否相关 ,其中PCNA表达与复发与否呈正相关 ,P <0 0 1。nm2 3表达与复发与否呈负相关 ,P <0 0 1。结论 :用阳性水平指数 ,作为免疫组化研究阳性表达水平指标 ,使研究由定性、半定量转化为定量 ,便于科学的统计分析。测定PCNA、nm2 3在膀胱移行细胞癌中的表达 ,具重要的临床指导意义。PCNA表达的阳性水平可用于肿瘤良恶性的鉴别。利用阳性水平指数 ,?Objective:To investigate the expression of PCNA and to nm23 and to determine their relationship with clinicopathological features in transitional cell carcinoma of bladder.Methods: Immunohistochemical technique,S-P method was used to study the expression PCNA and nm23 in 78 cases transitional cell carcinoma of bladder.Positive level index(PLI)was used to assess expressing level of PCNA and nm23 protein.The percentage of positive cell ,average optical density (AOD) was assessed by image analyzer and positive level index(PLI)=P/A(%)×AOD.Results:Overexpression of PCNA had been observed in transitional cell carcinoma of bladder.Expression PCNA had been observed in all 8 cases normal bladder mucosas.Statistical analysis revealed they had significant difference(P<0.01).Lower expression of nm23 had been observed in transitional cell carcinoma of bladder.Expression nm23 had been observed in all 8 cases normal bladder mucosas.Statistical analysis revealed they had significant difference(P<0.01).PCNA protein was associated with clinicopathological factors such as T-factor,stage,grade(P>0.01);Nm23 protein was not associated with clinicopathological factors such as T-factor,stage,grade in transitional cell carcinoma(P<0.05).Both of expression PCNA and nm23 protein were associated with relapse of bladder transitional cell carcinoma,of which PCNA was positively correlated with relapse of carcinoma,(P<0.01)and nm23 was negatively correlated with relapse of carcinoma,(P<0.01).Conclusions:PLI,as a reflector of immunohistochemical reaction,make the study of immunohistochemistry turn from quality,semi-quantity to quantity and make it easy to be statistically analyzed.Detecting the expression PCNA and nm23 protein in transitional cell carcinoma of bladder has more important meaning to clinical diagnosis and treatment.The expressing level of PCNA can be used to differentiate benign and malignant tumor.Using PLI,by analyzing big sample data,a criteria can be found to diagnose benign and malignant tumor.

关 键 词：PCNA 膀胱癌 免疫组化 基因表达 膀胱移行细胞癌 

分 类 号：R737.14[医药卫生—肿瘤]