凋亡基因Bcl-2、Bax、Fas、FasL在肝细胞癌中的表达  

The Expression of Bcl-2,Bax,Fas and FasL in Hepatocellular Carcinoma

作　　者：白玲[1] 张昶[1] 陈昊[1] 谭颖慧[2] 

机构地区：[1]江苏省连云港市第一人民医院病理科,连云港222002 [2]沈阳医学院医学基础部微生物教研室,沈阳110031

出　　处：《沈阳医学院学报》2001年第3期143-146,共4页Journal of Shenyang Medical College

摘　　要：目的　了解凋亡基因Bcl 2、Bax、Fas、FasL在肝细胞癌中的表达及意义。方法　免疫组化SP法 ,对 5 7例肝细胞癌组织、2 9例肝硬变组织进行染色 ,统计学处理采用 χ2 检验。结果　 5 7例肝细胞癌中的阳性率分别为 40 4%、89 5 %、5 0 9%、5 0 9%。 2 9例肝硬变组织中的阳性率分别为 6 9 0 %、86 2 %、86 2 %、86 2 %。其中Bcl 2、Fas、FasL在肝细胞癌组织中的表达明显低于肝硬变组织 (P<0 .0 5、P <0 .0 1、P<0 .0 1 ) ,而Bax则持续较高表达 ,且在肝细胞癌组织中Bax的表达与Bcl 2表达差异有高度显著性 (P <0 .0 1 ) ,Bax的阳性表达较Bcl 2敏感且广泛。在肝细胞癌组织中 ,上述基因表达水平与组织学分级之间无显著差异(P >0 .0 5 )。结论　Bax在肝细胞损伤、增殖及癌变过程中持续存在。肝细胞癌的发生可能与Bcl 2、Fas、FasL表达减少 ,Bcl 2与Bax的比例发生变化有关。Objective To understand the expression and significance of Bcl-2, Bax, Fas and FasL in hepatocellular carcinoma (HCC). Method S-P immunohistochemical method was used to examine the expression in the hepatocyte of 57 cases of Hepatocellular Carcinoma and 29 cases of Liver Cirrhosis (LC). Results The positive rates of Bcl-2, Bax, Fas and FasL were 40.4%, 89.5%, 50.9%, 50.9%, respectively, in 57 cases of HCC, and their positive rates were 69.0%, 86.2%, 86.2%, 86.2% respectively in 29 cases of LC. The expression of Bcl-2, Fas, FasL was more evident in LC than that in HCC (P<0.05, P<0.01,P<0.01). Bax was more evident than Bcl-2 in HCC (P<0.01). There was no statistic difference between the level of expression and histological classification in HCC (P>0.05). Conclusion The malignant transformation of hepatocyte may be correlate to decrease of Bcl-2, Fas and FasL, and it maybe relate to the variation of Bcl-2/Bax proportion.

关 键 词：肝细胞癌 肝硬变 Bcl-2 BAX FAS FASL 免疫组化 

分 类 号：R735.702[医药卫生—肿瘤]