非霍奇金淋巴瘤恶性程度与S期比值及DNA倍体关系  

Assessment of the Correlation between Histological Grade of Malignancy and its S-phase Cell Fraction,DNA Ploidy Patterns in non-Hodgkin′s Lymphoma

作　　者：谢贤和[1] 叶韵斌[1] 黄雪珍[1] 赵春利[1] 陈强[1] 陆俐丽[1] 吴允昆[1] 陈明水[1] 杨榆[1] 林银英[1] 

机构地区：[1]福建省肿瘤医院,福州市350014

出　　处：《四川肿瘤防治》2001年第3期138-140,共3页Sichuan Journal of Cancer Control

基　　金：福建省卫生厅科研基金资助(编号 :96039)

摘　　要：目的 :探讨非霍奇金淋巴瘤(NHL)恶性程度与S期比值、DNA倍体间的关系。方法 :取43例经组织学确诊的NHL(其中低度恶性12例 ,中度恶性7例 ,高度恶性24例)石蜡包埋标本 ,制成单细胞悬液 ,染色并行流式细胞术(FCM)分析 ,并对35例病人进行为期3年的随访。结果 :低度恶性NHL12例 ,S期比值均数为13.56%±6.89% ,中度恶性NHL7例 ,S期比值均数为34.55 %±26.20% ;高度恶性NHL24例 ,S期比值均数为31.93%±16.14% ;低恶组与中恶组相比 ,有显著性差异(P<0.05) ;低恶组与高恶组相比 ,有高度显著性差异(P<0.01) ;中恶组与高恶组相比 ,无显著性差异(P>0.05)。DNA倍体分析显示 ,43例NHL中 ,低度恶性者12例 ,全为二倍体 ;中度恶性者7例 ,二倍体6例 ,异倍体1例 ;高度恶性者24例 ,二倍体15例 ,异倍体9例 ;从低恶组至高恶组DNA异倍体率有显著性差异(P<0.05)。此外 ,对随访的35例病人 ,其中异倍体组9例 ,S期比值为38.65 %±16.72% ,有8例复发(其中1例于复发后6个月死于NHL) ;DNA二倍体组26例 ,S期比值为19.40±18.02 % ,有2例复发 ,二组病人复发率有高度显著性差异(P<0.01)。结论 :结合S期比值与DNA倍体分析 ,有助于NHL恶性程度的鉴别。Objective:To assess the correlation between histological grade of malignancy and S-Phase cell fraction,DNA ploidy patterns in non-Hodgkin′s lymphoma(NHL).Methods:From 43 cases with NHL(including 12 cases,7 cases and 24cases with low-grade,intermediate-grade and high-grade NHL respectively),their paraffin-embedded specimens,single-cell suspensions were prepared,stained and assessed by flow cytometer(FCM).Meanwhile,among 43 cases,35 cases with NHL were followed up for 3 years.Results:0 out of 12,1 out of 7 and 9 out of 24 cases showed DNA aneuploid in low-grade,intermediate-grade and high-grade NHL respectively;and the increasing of DNA aneuploid was observed from low-grade group up to high-grade group.Besides,it showed a positive correlation of DNA aneuploid between low-grade group and high-grade group.S-phase cell fractions in low-grade group,intermediate-grade group and high-grade group were 13.56±6.89%,34.55±26.20% and 31.93±16.14% respectively.It showed a positive correlation between low-grade group and intermediate-grade group or high-grade group.No significant correlation was found between intermediate-grade group and high-grade group.In addition,among 35 cases with NHL whom were followed up for 3 years,8 of 9 cases who appeared DNA aneuploidy and had high S-phase cell fractions developed clinical recurrence and one of them died within 6 months after recurrence; 2 of 26 cases who appeared DNAdiploid and had low S-phase cell fractions developed clinical recurrence.Conclusion:Our results show that it is helpful to differentiate the histological grade of NHL for predicting the early clinical relapse by detecting DNA ploidy pattern and S-phase cell fraction.

关 键 词：流式细胞术 非霍奇金淋巴瘤 S期比值 DNA倍体 

分 类 号：R733.1[医药卫生—肿瘤]