内毒素血症对大鼠肝细胞线粒体质子ATP酶的影响及中药912液的干预作用  

The effect of endotoxin on hepatic mitochondria H^+-ATPase synthesis and the therapeutic action of traditional Chinese medicine 912 in rats

作　　者：毕铭华[1] 张淑文[1] 王宝恩[1] 刘树森[2] 宋伟[2] 

机构地区：[1]北京友谊医院感染内科,北京100050 [2]中国科学院动物研究所生物膜与膜生物国家重点实验室

出　　处：《中国危重病急救医学》2001年第9期533-535,共3页Chinese Critical Care Medicine

基　　金：国家中医药管理局科研基金资助项目 (No.A类 165 )

摘　　要：目的 :观察内毒素血症模型大鼠肝细胞线粒体质子 ATP酶的损伤及中药 912液的保护作用。方法 :将大鼠随机分为正常对照组、内毒素组、912液组。提取内毒素血症大鼠肝细胞线粒体测定其超氧阴离子 (O÷2 )及丙二醛 (MDA)生成量 ,同时测定线粒体质子 ATP酶 ,并观察中药 912液的保护作用。结果 :内毒素血症大鼠肝细胞线粒体 O÷2 及 MDA量明显升高 ;肝细胞线粒体质子 ATP酶明显减少。经 912液治疗后大鼠肝细胞线粒体 MDA和 O÷2 量明显减少 ,线粒体质子 ATP酶明显增加。结论 :在内毒素血症的发病机制中存在由于肝细胞线粒体内源性氧自由基生成增加及其所引发的脂质过氧化反应增强对线粒体所造成的氧化损伤 ,中药 912液可以减少肝细胞线粒体内源性氧自由基生成 。Objective:To investigate the effect of endotoxin on hepatic mitochondria H +ATPase synthesis in rats and the therapeutic action of traditional Chinese medicine 912.Methods:A rat model of endotoxemia was used in this study.Animals were randomly divided into three groups:endotoxin group,912 treatment group and the normal controls.The liver mitochondria H +ATPase activity,production of endogenous oxygen free radicals (O ÷ 2) and malonyldialdehyde (MDA) levels were determined.Results:The significantly increase in O ÷ 2 and MDA levels were observed in liver mitochondria following endotoxin challenge ( P <0 01).Meanwhile,liver mitochondria H +ATPase synthesis in endotoxin group significantly decreased ( P <0 01).In the 912 group,liver mitochondria MDA and O ÷ 2 levels were markedly lower but H +ATPase synthesis significantly higher than those in endotoxin group ( P <0 01).Conclusions:Endotoxin,which stimulates the production of endogenous oxygen free radicals,plays an important role in hepatic mitochondria dysfunction in rats.Treatment with traditional Chinese medicine 912 may have beneficial effect on endotoxininduced acute liver injury.

关 键 词：线粒体 肝细胞 内毒素血症 ATP酶系统 氧化损伤 中药912液 

分 类 号：R363[医药卫生—病理学]