大鼠坐骨神经损伤后背根节感觉神经元神经丝蛋白基因表达的变化  

ALTERED EXPRESSION OF NEUROFILAMENT GENES IN RAT SENSORY NEURONS OF THE DORSAL ROOT GANGLION AFTER SCIATIC NERVE CRUSH

作　　者：米瑞发[1] 周长满[1] 范明[2] 

机构地区：[1]北京军医学院,北京100071 [2]军事医学科学院基础医学研究所,北京100850

出　　处：《解剖学报》2001年第4期324-328,共5页Acta Anatomica Sinica

基　　金：国家 973资助项目 (G19990 5 40 0 0 )

摘　　要：目的　探讨神经损伤再生过程中细胞骨架蛋白基因表达调控机制。　方法　用原位杂交组织化学技术观察了大鼠坐骨神经夹伤后神经再生过程中 ,L4～ 6 背根节感觉神经元内轻分子量 (light,L)、中分子量 (medium ,M)和重分子量 (heavy ,H)神经丝蛋白亚基mRNA的表达变化。　结果　光镜下 ,轴突损伤后背根节神经元内各神经丝亚基mRNA的杂交信号明显减弱 ,神经丝 L和神经丝 MmRNA的杂交信号位于神经元胞浆内 ,而在神经元胞浆和胞核内均可见神经丝 HmRNA的表达信号。　结论　神经丝 3个亚基基因表达的调控机制不同 ,神经丝基因表达的下调可能是神经元对轴突损伤的基本应答 。Objective In order to explore the mechanisms that regulate the expression of genes coding for cytoskeletal proteins during nerve regeneration. Methods In the present study, in situ hybridization was used to examine the changes of light(NF L),medium(NF M)and heavy (NF H)neurofilament protein subunits mRNA in L 4-6 dosal root ganglion (DRG) sensory neurons during nerve regeneration following a unilateral crush of the sciatic nerve. Results The hybridization signals of each neurofilament subunit mRNA were dramatic decrees in DRG sensory neurons post axotomy by light microscope.The signals of NF\|L and NF\|M mRNA were located in cytoplasm of neurons,whereas NF H mRNA was found in both nucleus and cytoplasm of neurons. Conclusion There are the different mechanisms of regulation of neurofilament subunit genes expression,the reduced neurofilament gene expression may represent a general response to axonal injury and plays an important role in effective nerve regeneration.

关 键 词：神经丝蛋白 mRNA 背根节神经元 原位杂交 神经再生 大鼠 坐骨神经损伤 

分 类 号：R745.42[医药卫生—神经病学与精神病学] R322.81[医药卫生—临床医学]