应用间接ELISA技术快速检测花鲈病原菌-鳗弧菌  

An Indirect ELISA Technique for Rapid Detection of Vibrio anguillarum in Sea perch ( Lateolabrax japonicus )

作　　者：余俊红[1] 姚斐[1] 王宝坤[1] 纪伟尚[1] 徐怀恕[1] 

机构地区：[1]青岛海洋大学生命学院,青岛266003

出　　处：《高技术通讯》2001年第7期23-27,共5页Chinese High Technology Letters

基　　金：国家自然科学基金 (3 9870 5 81); 973计划 (G1999.0 12 .0 0 4)资助项目

摘　　要：以花鲈 (Lateolabraxjaponicus)弧菌病的病原菌———鳗弧菌 (Vibrioauguil larum)W 1为抗原 ,制备兔抗血清 ;利用辣根过氧化酶标记的羊抗兔血清 (HRP IgG)为酶标二抗 ,建立了检测鳗弧菌的间接ELISA快速检测法。结果表明 ,应用间接ELISA技术检测鳗弧菌有较高的灵敏度 ,最低的检测量为 10 5个 /ml,即 10 4 个 /孔。同时交叉反应表明其具有较高的特异性。对 4 6份人工感染后的花鲈组织样品 ,包括肌肉、鳃、肠、肝、肾等组织的检测表明 ,阳性检测率为 76 .1%。An indirect enzyme linked immunosorbent assays (ELISA) was developed for the rapid detection of Vibrio anguillarum in sea perch ( Lateolabrax japonicus ) which infected artificially. The ELISA, which incorporated a polyclonal antiserum produced in a male New Zealand white rabbit, detected 10 5 cells of V. anguillarum /ml. The systems permitted the recognition of V. anguillarum isolates, but not those of other taxa. The specificity and sensitivity antiserum were test on tissues which including muscle, hinder gut, gill, liver and kidney from sea perch infected artificially with viable W 1 ( V. anguillarum ).The results showed 76.1% of 46 samples were positive by indirect ELISA detection.

关 键 词：ELISA技术 鳗弧菌 检测 花鲈 病原菌 酶联免疫吸附试验 海水鱼 弧菌病 兔抗血清 

分 类 号：S941[农业科学—水产养殖]