Fos蛋白参与牙髓炎疼痛中枢调控作用的实验研究  

The Role of Fos Protein in Modulation of Dental Pain inCentral Nerve System

作　　者：林正梅[1] 程斌[1] 凌均棨[1] 陈小华[1] 刘建伟[1] 

机构地区：[1]中山医科大学口腔医学院附属光华口腔医院口腔内科,510055

出　　处：《华西口腔医学杂志》2001年第4期253-255,259,共4页West China Journal of Stomatology

基　　金：中山医科大学科研基金资助项目 (编号 960 15 )

摘　　要：目的 :研究大鼠实验性牙髓炎疼痛诱导Fos蛋白表达与其靶基因脑啡肽前体mRNA转录、脑啡肽水平改变之间的关系 ,探讨牙痛的中枢调节机制。方法 :应用免疫组化方法观察Fos蛋白在三叉神经脊束核尾侧亚核 (sp5c)内的表达、原位杂交技术检测PENKmRNA的转录和放射免疫技术测定sp5c内ENK含量的变化。结果 :实验性牙髓炎疼痛诱发Fos蛋白在sp5c内的表达呈时间依赖性 ,Fos蛋白在诱痛后 0 5h表达 ,2h达高峰 ,4h后渐减弱 ;PENKmRNA在牙痛后 2h始转录 ,4h达高峰 ,8h后渐减弱。牙髓炎诱痛后 4hsp5c内ENK含量显著升高 (P <0 0 1)。结论 :Fos蛋白通过激活靶基因脑啡肽前体基因的转录 ,引起中枢内脑啡肽水平的升高而参与牙痛的中枢调节。Objective:The aims of this study were to reveal the relationship between Fos protein expression, the transcription of preproenkephalin (PENK) mRNA, and the change of enkephalin (ENK) level initiated by experimental dental pain, and to understand the central modulation mechanism of dental pain. Methods:The techniques of Immunohistochemistry in situ hybridization and radioimmunoassay were applied respectively to detect the expression of Fos, the transcription of PENK mRNA, and the change of ENK level in the experimental nucleus of the spinal tract of the trigeminal nerve, caudal part (sp5c) of rats.Results:It was found that Fos protein expression was in a time_dependant manner. Fos appeared half hour after the pain stimulation, and its peak occurred two hours after the pain stimulation, but disappeared slowly four hours later. The transcription of PENK mRNA was seen two hours after the pain stimulation, its peak appeared four hours later and it disappeared eight hours later. The level of ENK increased significantly (P<0.01) in the caudal part of the spinal tract of the trigeminal nerve four hours after pain stimulation.Conclusion:Fos protein may take a part in the central modulation of dental pain by initiating PENK mRNA transcription which led to the increment of ENK.

关 键 词：牙痛 FOS蛋白 脑腓肽前体基因 脑啡呔 牙髓炎 中枢调节 RNA 

分 类 号：R781.31[医药卫生—口腔医学]