免疫印迹法检测肝纤维化组织基质金属蛋白酶抑制因子-1表达水平  

Tissue inhibitor of metalloproteinase-1 protein expression is enhanced in experimental liver fibrosis

作　　者：黄宇琦[1] 魏银燕 高毅[3] 李朝龙[1] 

机构地区：[1]第一军医大学南方医院肝胆外科,广州510515 [2]第一军医大学校办,广州510515 [3]珠江医院肝胆外科,广州510282

出　　处：《广东医学》2001年第7期569-570,共2页Guangdong Medical Journal

基　　金：广东省自然科学基金资助课题 (编号 :960 362 )

摘　　要：目的　观察实验性肝纤维化过程中金属蛋白酶组织抑制因子 1(TIMP 1)蛋白表达水平的动态变化。方法　建立四氯化碳中毒性大鼠肝纤维化模型 ,采用免疫印迹方法 (Westernblot法 ) ,测定肝纤维化不同阶段TIMP 1的蛋白表达水平。结果　正常大鼠肝组织可见TIMP 1的蛋白表达。在肝纤维化的发生、发展过程中 ,TIMP 1蛋白表达逐渐增强 ,到肝硬化阶段达到高峰。结论　TIMP 1可能是促进和影响肝纤维化。To investigate the dynamic alteration of tissue inhibitor of metalloproteinase 1(TIMP 1) protein expression in experimental liver fibrosis. Methods CCl 4 induced animal models of liver fibrosis was established and TIMP 1 protein expression level in different stages of liver fibrosis was measured by Western blot. Results TIMP 1 protein expression was detected in normal rat liver.TIMP 1 protein expression level was increased gradually in the development of liver fibrosis, reached its peak at the stage of liver cirrhosis. Conclusion TIMP 1 may be an important factor in promoting liver fibrosis and cirhosis.

关 键 词：金属蛋白酶 组织抑制因子-1 蛋白表达 实验性 肝纤维化 ECM MMPS 免疫印迹法 

分 类 号：R575.2[医药卫生—消化系统] R446.61[医药卫生—内科学]