骨形成蛋白2基因转染的人牙周膜成纤维细胞超微结构观察  

The ultrastructure of bone morphogenetic protein 2 gene transfected human period ontal ligament fibroblasts

作　　者：司晓辉[1] 刘正[1] 

机构地区：[1]上海第二医科大学口腔医学院口腔内科,200011

出　　处：《中华口腔医学杂志》2001年第4期266-268,共3页Chinese Journal of Stomatology

基　　金：国家自然科学基金 (3 0 0 0 0 191);中国博士后科学基金(1999第 17号 );上海市博士后基金 (沪科 2 0 0 0第 487号 )资助项目

摘　　要：目的　观察稳定表达骨形成蛋白 2 (bonemorphogeneticprotein 2 ,BMP 2 )的人牙周膜成纤维细胞 (humanperiodontalligamentfibroblasts ,HPDLFs)的超微结构。方法　用脂质体转染法将BMP 2表达载体转染至HPDLFs ,以原位杂交和免疫组化法检测BMP 2的稳定表达 ,透射电镜观察细胞的超微结构。结果　转染BMP 2基因的HPDLFs内质网池普遍扩张 ,细胞内基质膜泡和髓鞘样结构明显增加 ,细胞间广泛分布胶原原纤维。结论　转染BMPObjective To observe the ultrastructure of bone morphogenetic protein 2 (BMP-2) gene tran sf ected human periodontal ligament fibroblasts (HPDLFs). Methods An expression vector for BMP-2 was transfected into HPDLFs by using Lipofect AM INE. The stable expression of BMP-2 was determined by in situ hybridization a nd immunohistochemistry. The ultrastructure of the transfected cells was examine d by transmission electron microscope. Results The results showed that the BMP-2 gene transfected cel ls showed expansive endoplasmic reticulum, incremental myelin sheath-like figures and matrix vesicles. The collagenous fibrillae was distributed widely intercell ular. Conclusions The results suggests that BMP-2 gene transfe ction tends to accelerate HPDLFs into osteoblast-like cells.

关 键 词：成纤维细胞 骨形成蛋白 BMP HPDLFs 牙齿 细胞生物 

分 类 号：R780.2[医药卫生—口腔医学]