口腔粘膜疣状癌基膜的免疫组化和超微结构研究  

Immunohistochemical and ultrastructural study of basement membrane in oral verrucous carcinoma

作　　者：蒋玲[1] 汪说之[1] 陈新明[1] 

机构地区：[1]武汉大学口腔医学院病理科,430079

出　　处：《中华口腔医学杂志》2001年第4期308-310,共3页Chinese Journal of Stomatology

摘　　要：目的　研究口腔粘膜疣状癌基膜Ⅳ型胶原与层粘连蛋白的表达、分布和超微结构改变。方法　采用免疫组化S P法 ,分别检测 16例疣状癌、10例鳞癌和 9例中至重度不典型增生病例中Ⅳ型胶原与层粘连蛋白的表达 ,对表达结果作定量分析 ,并对 3例疣状癌作电镜观察。结果　疣状癌基膜大多数较厚且保持完整 (13/ 16 ) ,电镜下观察基板在一些区域明显增厚。但炎症细胞较多的病例 ,基膜出现中断 (3/ 16 )。鳞癌和中至重度不典型增生基膜相对较薄 ,鳞癌基膜绝大多数不连续 (9/ 10 ) ,尤其在浸润的前沿或小条索状癌结构中 ,基膜基本中断或消失。中至重度不典型增生基膜大多数连续 (6 / 9)。所有病例的癌巢周围间质中都可见炎症细胞浸润 ,疣状癌较鳞癌和中至重度不典型增生中浸润的淋巴细胞更为密集 (P <0 0 5 )。在疣状癌中 ,上皮内炎症细胞浸润与其基膜完整呈正相关关系 (P <0 0 1)。结论　疣状癌基膜较厚 ,较完整且结缔组织中有大量以淋巴细胞为主的炎症细胞浸润 。Objective To study the immunohistochemical alteration of basement membrane (BM) typeⅣ col lagen and laminin in oral verrucous carcinoma and its BM ultrastructural variati ons Methods 16 cases of oral verrucous carcinoma (OVC), 10 cases of oral squamous cell carcinoma (OSCC) and 9 cases of oral mild to severe epithelial dysplasia (OMSD) were studied by using a immunohistochemical S-P me thod, and the results were analyzed by quantitative method 3 cases of OVC were observed by TEM Results The BM in OVC was thicker than in OS CC and OMSD TEM found the basal lamina in some areas showed a marked reduplica tion The BM in OVC was generally intact (13/16), whereas in OSCC it was mostly discontinuous (9/10), especially around the neoplasm front or the small cord co nsisted of a few cells, and mostly continuous in OMSD (6/9) There was a stroma l inflammatory infiltration around tumor nests for all the oral lesions, but it was much heavier in OVC than that in OSCC and OMSD( P<005) There was a positive correlation between intraepithelial lymphocytic infiltration and the BM continuity for OVC( P<001) Conclusions The more c o ntinuous BM and the heavier inflammatory infiltration in the connective tissue o f OVC may be related to its biological behavior

关 键 词：癌 鳞状细胞 胶原 免疫组织化学 疣状癌 口腔粘膜 

分 类 号：R739.85[医药卫生—肿瘤] R730.21[医药卫生—临床医学]