Nested RT-PCR方法测定PSM诊断前列腺癌的临床意义  

Diagnostic value of PSM on prostate carcinoma

作　　者：李青[1] 王振声[1] 李广云[1] 杨波[1] 魏学斌[1] 

机构地区：[1]山东省千佛山医院,山东济南250014

出　　处：《山东医药》2001年第13期13-14,共2页Shandong Medical Journal

基　　金：山东省卫生厅科研课题(199;CAIDCB4)

摘　　要：采用巢式逆转录聚合酶链（ｎｅｓｔｅｄ ＲＴ－ＰＣＲ）方法，对３１例前列腺癌患者和３５例对照组的外周血及 前列腺癌组织中的前列腺特异膜抗原（ＰＳＭ）ｍＲＮＡ的表达进行测定。结果显示，前列腺癌组外周血ＰＳＭｍＲＮＡ 术前总检出率为６１．２９％．术后为５８．０６％；３例骨转移者为阳性。前列腺癌组织均为阳性。对照组ＰＳＭｍＲＮＡ均 为阴性。认为ＰＳＭ检测有助于前列腺癌的早期诊断和治疗．对预测其转移和血行播散具有特异性和敏感性。PSMmRNA expression in peripheral blood and prostate carcinoma tissue of 31 prostate carcinoma cases and 35 control cases were determined by nested RT-PCR procedure. Result showed that the preoperative positive rate of PSMmRNA in peripheral blood of prostate carcinoma cases was 61.29%,afteroperative positive rate was 58.06%,PSMmRNA in prostate carcinoma tissue was positive and in control cases was negtive.PSM is valuable for diagnosis and treatment of prostate carcinoma in early stage,and forecasting the metastasis and blood dissemination, with more sensitivity and specificity.

关 键 词：前列腺癌 膜抗原 诊断 RG-PCR 巢化逆转录聚合酶链 PSM 

分 类 号：R737.25[医药卫生—肿瘤] R730.43[医药卫生—临床医学]