人膀胱移行细胞癌HPV-18感染与G_1-细胞周期素表达的关系  

Relationship of Infection of Human Papillomaviruses Type 18 and G_1-cyclins Expression in Transitional Cell Carcinoma of Human Bladder

作　　者：郑闪[1] 何祖根[1] 刘海涛 王顺宝[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤研究所肿瘤医院病理科,北京100021

出　　处：《癌症》2001年第7期709-712,共4页Chinese Journal of Cancer

基　　金：国家自然科学基金资助项目(编号39370291)

摘　　要：目的：探讨人膀胱移行细胞癌(transitional cell carcinoma，TCC)组织内人乳头瘤病毒18型（human papillomavirus type 18，HPV-18）DNA与G_1-细胞周期素（D1和E）表达之间的关系。方法：用免疫组织化学方法检测57例人膀胱TCC组织（PCR测定29例HPV-18 DNA阳性、28例阴性）及对照组(7例正常膀胱)G_1-细胞周期素的表达。结果：细胞周期素D1在癌组织中表达的阳性率71.93％(41/57)；HPV-18 DNA阳性组阳性率55.17％(16/29)，阴性组阳性率89.29％（25/28），阳性组表达率较阴性组低，两者表达有差异(P< 0.05)；与对照组14.29％（1/7）相比，癌组织表达明显增高(P< 0.05)。细胞周期素E在癌组织中表达阳性率为63.16％(36/57)，在HPV-18 DNA阴性（67.86％）和阳性组（58.62％）之间无明显差异(P >0.05)；与对照组(0％)相比，癌组织表达明显增高(P< 0.05)。结论：G_1-期细胞周期素的改变与膀胱移行细胞癌的发生相关。HPV-18 DNA与细胞周期素D1表达存在显著的负相关。Objective：This study was designed to investigate relationship between the G_1-cyclins(D1 and E) expression and the DNA of human papillomaviruses type 18 (HPV-18) in the tissues of the transitional cell carcinoma (TCC) of human bladder. Method：Immunohistochemistry was used in the detection of the expression of G_1-cyclins in 57 TCC (PCR confirmed 29 cases to be HPV-18 DNA positive, and 28 cases negative) and in the control(7 normal bladders). Result：The positive rate of the expression of cyclin D1 in the TCC was 71.93％(41/57)；the positive rate of the expression of cyclin D1 was 55.17％(16/29), in HPV-18 DNA positive group and 89.29％(25/28). in negative group There was obvious difference(P< 0.05) between TCC HPV-18 DNA positive group and negative group, which was higher in the HPV-18 DNA negative group than in the HPV-18 DNA positive group. The expression rate of cyclin D1 was higher in the TCC than that in the control group with an expression rate of 14.29％(P< 0.05). The positive rate of the expression of cyclin E in the TCC was 63.16％(36/57). The expression rates of HPV-18 DNA positive and HPV-18 DNA negative were 58.62％and 67.86％respectively, and there was no difference between two groups. There was obvious difference in the expression of cyclin E(P< 0.05) between the TCC and the normal control, and the expression rate of the latter was 0％. Conclusion：The change in G_1-cyclins correlates with the carcinogenesis of TCC of uninary bladder and HPV-18 DNA is negatively correlated with the expression of cyclin D1.

关 键 词：膀胱肿瘤 人乳头瘤病毒 细胞周期素 免疫组织化学 聚合酶链式反应 

分 类 号：R737.14[医药卫生—肿瘤]