苯基棕榈酰胺吗啡丙醇对卵巢癌多药耐药调节的实验研究  

Modulation of multidrug resistance by 1-phenyl-2-palmitoylamino-3-morpholino-1-propanol in SKOV3-adriamycin-resistant cell line

作　　者：袁匀[1] 王丽莉[1] 张积仁[2] 

机构地区：[1]第一军医大学附属珠江医院妇产科,广州510282 [2]第一军医大学附属珠江医院肿瘤中心,广州510282

出　　处：《中华妇产科杂志》2001年第8期493-495,共3页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目 ( 3970 0 14 3;39870 813)

摘　　要：目的　研究葡糖酰基鞘氨醇合酶抑制剂苯基棕榈酰胺吗啡丙醇 (PPMP)对耐阿霉素卵巢癌细胞亚株SKOV3 AdrR的多药耐药基因mdr1及P 糖蛋白的调节。方法　应用细胞培养技术对SKOV3 AdrR细胞进行PPMP处理 ,采用逆转录 聚合酶链反应技术分析mdr1mRNA的表达 ,流式细胞仪检测细胞内罗丹明的荧光强度。结果　浓度为 5、15 μmol L的PPMP可部分抑制SKOV3 AdrR细胞mdr1mRNA的表达 ,2 5 μmol L的PPMP可完全抑制SKOV3 AdrR细胞mdr1mRNA的表达 ,调节作用呈浓度依赖性。PPMP可增加SKOV3 AdrR细胞内罗丹明的荧光强度 ,随着PPMP浓度 (分别为 5、15、2 5μmol L)的增加 ,细胞内罗丹明的荧光强度逐渐增高 (分别为 3 0 1 2 2、3 89 98、4 2 6 0 8)。 结论　PPMP可逆转SKOV3 AdrR的多药耐药性。Objective To study the modulation of mdr1 and P glycoprotein (P gp) by 1 phenyl 2 palmitoylamino 3 morpholino 1 propanol (PPMP) in SKOV3 adriamycin resistant (SKOV3/AdrR) cell line Methods SKOV3/AdrR cells were treated with PPMP, mRNA expression of multidrug resistant (mdr1) gene was analyzed by reverse transcriptase polymerase chain reaction Intracellular rhodamine (Rh123) concentration was measured by flow cytometry Results PPMP was found to inhibit mdr1 expression of SKOV3/AdrR at the mRNA level This modulation of gene expression was content dependent and complete inhibition appeared at 25 μmol/L PPMP treatment PPMP could increase intracellular Rh123 accumulation in SKOV3/AdrR cells After 15, 25 μmol/L PPMP treatment, Rh123 accumulation in SKOV3/AdrR was markedly enhanced Rh123 fluorescence intensity were 389 98,426 08 respectively ( P <0 05) Conclusions PPMP could modulate mdr1 expression at the mRNA level in a content dependent manner P gp mediated outward efflux of cytotoxic agents can be blocked by PPMP in SKOV3/AdrR cell PPMP possesses multi drug resistance activity in ovarian cancer

关 键 词：吗啡类 鞘脂类 MDR基因 P-糖蛋白 逆转录聚合酶链反应 流式细胞术 卵巢癌 

分 类 号：R737.31[医药卫生—肿瘤]