人原发性肝细胞癌中热休克蛋白70mRNA的表达  

Expression of heat shock protein 70 mRNA in human primary hepatocarcinoma

作　　者：胡沛臻[1] 杨守京[1] 马福成[1] 刘彦仿[1] 

机构地区：[1]第四军医大学西京医院病理科,西安710032

出　　处：《诊断病理学杂志》2001年第4期220-221,共2页Chinese Journal of Diagnostic Pathology

基　　金：国家自然科学基金 ( 39770 664 )

摘　　要：目的　检测热休克蛋白 70mRNA在人原发性肝癌中的表达情况 ,探讨热休克蛋白 70在原发性肝癌演变过程中的意义。方法　采用原位杂交技术 ,用地高辛标记的热休克蛋白 70cDNA探针检测 4 1例人原发性肝癌组织及2 0例肝硬变组织。结果　原位杂交结果显示 4 1例人原发性肝癌组织中 2 1例热休克蛋白 70mRNA阳性 ( 5 1 2 % ) ,2 0例肝硬变组织有 11例阳性 ( 5 5 0 % ) ,两组之间无显著差异 (P >0 0 5 )。结论　热休克蛋白 70mRNA在人原发性肝癌组织中及肝硬变组织中有较高水平的表达 ,提示热休克蛋白 70参与了原发性肝癌的发生及发展过程 ,对其生物学特性作进一步研究 ,可能为肝癌发病机制提供一定的理论依据。Objective To study the expression of heat shock protein 70 (hsp70) mRNA in human primary hepatocarcinoma (PHC) and to explore the role of hsp70 in the histogenesis of PHC. Method In situ hybridization was used to detect hsp70 mRNA distribution in the formalin-fixed and paraffin-embedded tissues from 41 cases of PHC and 20 liver cirrhosis. Results Expression of hsp70 was found in 21 in 41 cases of PHC (51.2%) and 11 in 20 cases of liver cirrhosis (55.0%). There was no significant correlation between the two groups in the expression of hsp70 (p>0.05). Conclusion The fact that hsp70 mRNA is highly expressed in PHC and liver cirrhosis suggests that hsp70 may involve in the histogenesis of PHC.

关 键 词：肝肿瘤 热休克蛋白70 原位杂交 MRNA 

分 类 号：R735.7[医药卫生—肿瘤]