聚丙烯酰胺凝胶电泳部分纯化新生牛肝再生刺激因子  

Partial Purification of Hepatic Stimulator Substance by Polyacrylamide Gel Electrophoresis

作　　者：余环 柳 陈章林 

机构地区：[1]江西省医科所病毒生化室

出　　处：《江西医学院学报》1995年第3期19-21,F003,共4页Acta Academiae Medicinae Jiangxi

摘　　要：利用新生牛肝脏，采用改良Ｌａｂｒｅｃｑｕｅ法提取肝再生刺激因子（ＨＳＳ），并应用非解离聚丙烯酰胺凝胶电泳对其进行纯化，ＳＤＳ－聚丙烯酰胺凝胶电泳证实其分子量约为１５０００道尔顿。经过纯化，其杂蛋白含量降低近９５０倍。Hepatic stimulator substance(HSS)was extracted from new born calf liver with themodified method of Labrecque,and purified by nondissociating polyacrylamide gel elec-trophoresis(PAGE).The molecular weight of HSS determined with the method of SDS-PAGE was 15,000 Dalton. After purification,the content of protein decreased about 950folds.

关 键 词：肝再生刺激因子 聚丙酰凝胶 生物化学 电泳纯化 肝功能衰竭 

分 类 号：R575.2[医药卫生—消化系统] Q503[医药卫生—内科学]