离散因子/肝细胞生长因子cDNA转染对肝癌SMMC7721细胞生长和转移的影响  

Influence of scatter factor/hepatocyte growth factor on the growth and transmission of hepatocarcinoma SMMC 7721 cells

作　　者：谢倩[1] 刘康达[1] 胡美玉[1] 吴祥甫[1] 周康[1] 

机构地区：[1]上海医科大学中山医院实验研究中心,200032

出　　处：《中华肝脏病杂志》2001年第2期95-97,共3页Chinese Journal of Hepatology

基　　金：国家自然科学基金!(39970290)

摘　　要：目的　通过离散因子／肝细胞生长因子（ｓｃａｔｔｅｒ ｆａｃｔｏｒ／Ｈｅｐａｔｏｃｙｔｅ ｇｒｏｗｔｈ ｆａｃｔｏｒ，ＳＦ／ＨＧＦ）ｃＤＮＡ转染肝癌ＳＭＭＣ７７２１细胞来探讨ＳＦ／ＨＧＦ对肝癌生长和转移的影响。方法　用脂质体法进行基因转染，以ＥＬＩＳＡ和ｗｅｓｔｅｒｎ ｂｌｏｔ检测ＳＦ／ＨＧＦ及其受体ｃ－ｍｅｔ的表达，通过生长曲线、划痕实验比较转染前后细胞的增长状况及细胞的运动能力。以转染前后细胞分别接种裸鼠，观察移植瘤的生长及转移情况。结果　转染后细胞表达ＳＦ／ＨＧＦ的量为６９４ｐｇ／ｍｌ，其受体ｃ－ｍｅｔ量未见明显变化；转染后细胞增殖明显加快，运动能力增强，并伴明显的形态学变化。初步动物实验显示转染后细胞生长较快，瘤组织中有瘤栓形成，并在肺内发现转移灶。结论　ＳＦ／ＨＧＦ在肝癌细胞中的高表达能促进肝癌细胞生长、侵袭及转移。Objective: To explore the influence of scatter factor/hepatocyte growth factor (SF/HGF) on the growth and transmission of hepatocarcinoma SMMC 7721 cells by SF/HGF cDNA transfection. Methods Gene transfection was operated by lipofectin method. In vitro, SF/HGF and c-met expression was tested by ELISA and Western blot. Cell proliferation and motility were compared by growth curves and wound healing assays. In vivo, cells before and after transfection were implanted subcutaneously into inde mice respectively to observe tumor growth and metastasis. Results After transfection, the expression of SF/HGF reached 694 pg/ml, compared with 0.26 pg/ml before transfection; however the expression of c-met protein did not change obviously. Growth curves showed that cells after transfection proliferated more rapidly than those before transfection and had an increased capability of motility together with enlarged morphological changes. In vivo experiment, tumors originated from SF/HGF (+) cells grew faster than those from SF/HGF (-) cells and had embolism formed inside and metastasis in the corresponding lung tissues, whereas no such findings in SF/HGF (-) cell originated tumors. Conclusions High expression of SF/HGF can stimulate tumor cell proliferation and metastasis in hepatocellular carcinoma.

关 键 词：肝细胞生长因子 转染 肝细胞癌 肿瘤转移 

分 类 号：R735.7[医药卫生—肿瘤]