喉癌端粒酶活性检测及其与颈淋巴结转移的相关性  

Detection of telomerase activity in laryngeal carcinoma and the association between telomerase activity and lymphatic metastasis

作　　者：吕秋萍[1] 吕飒音[2] 王明善[1] 叶非常[1] 刘认华[1] 祝怀平[3] 戴海明[3] 

机构地区：[1]安徽省立医院耳鼻咽喉科,合肥230001 [2]安徽大学实验中心 [3]安徽省立医院中心实验室

出　　处：《临床耳鼻咽喉科杂志》2001年第8期360-362,共3页Journal of Clinical Otorhinolaryngology

基　　金：安徽省卫生厅资助项目 (No.A95 - 1998- 2 5 )

摘　　要：目的 :探讨喉鳞状细胞癌端粒酶活性表达及其与颈淋巴结转移的相关性。方法 :采用多聚酶链反应 -酶联免疫吸附法 (PCR- EL ISA) ,对 47例喉鳞状细胞癌组织及 10例声带炎性息肉组织的端粒酶活性进行定量检测。结果 :47例喉鳞癌组织中 39例端粒酶呈阳性 (83% ) ,而 10例声带炎性息肉组织均为阴性 ,两组差异有极显著性意义 (P <0 .0 1) ;端粒酶活性在伴有颈淋巴结转移的喉癌中显著高于无淋巴结转移者 (P <0 .0 5 )。结论 :端粒酶在喉癌组织中有较高的表达 ,可作为喉癌的肿瘤标志物。喉癌组织端粒酶活性有可能作为预测喉癌转移和预后的指标 ,并可望指导临床有针对性地应用选择性颈廓清术。Objective:To investigate the telomerase activity in human laryngeal squamous cell carcinomas (LSCC) and the association between telomerase activity and lymphatic metastasis.Method:Telomerase activity was determined in 47 specimens of LSCC and 10 specimens of laryngeal inflammatory polyps using PCR ELISA.Result:Telomerase activity was detected in 39 of 47 (83%) LSCC,but was not detected in all 10 laryngeal inflammatory polyps.There were significant differences between them (P< 0.01 ).Telomerase activation in LSCC with positive cervical lymph node involvement appeared to be higher (13 out of 13 cases,100%) than in LSCC with negative cervical lymph node involvement (26 out of 34, 76.47 %),(P< 0.05 ).Conclusion:Telomerase is activated frequently in LSCC and may become a biomarker of LSCC.The detection of telomerase activity in LSCC may have some value for predicting metastasis potential and prognosis and can guide the clinical practices applying selective neck dissection.

关 键 词：喉肿瘤 端粒酶 淋巴转移 聚合酶链反应 酶联免疫吸附测定 

分 类 号：R739.650.6[医药卫生—肿瘤] R733.4[医药卫生—临床医学]