血小板对单核细胞促凝活性的影响  

Effect of platelet on procoagulant activity of monocyte

作　　者：许建平[1] 何晓凡[1] 熊石龙[1] 李俊成[1] 贺石林[1] 

机构地区：[1]中南大学湘雅医学院止血生理实验室,长沙410078

出　　处：《中华血液学杂志》2001年第9期478-480,共3页Chinese Journal of Hematology

基　　金：国家自然科学基金资助项目 ( 39830 180 )

摘　　要：目的　研究血小板对单核细胞促凝活性的影响。方法　采用一期法血浆凝固时间测定单核细胞促凝活性 ;单抗中和法确定组织因子 (TF)活性及P 选择素的作用。结果　①内毒素激活的单核细胞促凝活性显著增高 ,TF单抗能明显阻断该效应 ;②胶原激活的血小板对单核细胞及内毒素激活的单核细胞的促凝活性均有明显的增强作用 ;③抗血小板P 选择素单抗能阻断活化的血小板对单核细胞促凝活性的增强效应。结论　①单核细胞促凝活性是由于TF的表达 ;②血小板对单核细胞促凝活性的增强效应是通过P 选择素实现的。Objective To investigate the effect of platelet on procoagulant activity of mononcyte(M). Methods The procoagulant activity of M lysates was measured by one stage clotting time assay, the tissue factor (TF) activity and the role of P selectin were confirmed by specific monoclonal antibodies (McAb). Results ① Activated M challenged by lipopolysaccharide(LPS) had augmented procoagulant activity and this effect was blocked by TF McAb. ②Procoagulant activity of M and activated M increased significantly with the presence of activated platelets. ③The enhancement effect of activated platelet on the procoagulant activity of M was blocked by P selectin McAb. Conclusion TF expression of M contributes to the procoagulant activity of Ms and activated platelet enhances the procoagulant activity via P selectin.

关 键 词：单核细胞 血小板 组织因子 P-选择素 单核细胞促凝活性 

分 类 号：R331.1[医药卫生—人体生理学]