人食管癌及癌前上皮p53基因的表达  

Expression of Wild-type p53 mRNA in Human EsophagealCarcinoma and Premalignant Epithelia

作　　者：游绍进[1] 沈忠英[1] 吕丽春 

机构地区：[1]汕头大学医学院病理教研室

出　　处：《汕头大学医学院学报》1995年第1期5-8,共4页Journal of Shantou University Medical College

摘　　要：采用Ｄｉｇ标记探针原位杂交组化方法，对２０例食管癌及癌旁卷切粘膜组织切片中ｐ５３基因（Ｗｉｌｄ－ｔｙｐｅｐ５３，ｗ－ｐ５３）ｍＲＮＡ水平的表达进行了研究。结果表明从上皮形态正常到鳞状细胞癌等一系列病变中，ｐ５３ｍＲＮＡ水平的表达始终是低水平的，尽管在中、重度异型增生、原位癌及高分化鳞癌中有所增强。这可能是ｐ５３基因最终未能抑制食管上皮恶性转化的原因之一。We have studied the transcription of wild-type p53 (w-p53) gene using in situ hybridization histochemical method by Dig labeled probe in 20 cases of esophageal carcinoma and the mucosa adjacent to the cancer.Our results revealed that w-p53 mRNA was expressed at low level through the whole multistep processes of carcinogenesis from nomal epithelia to squamous cell carcinoma,although moderately increased expression of the gene was seen in the serious dysplasia epithelia,in situ and well-differentiated carcinoma. This might be one reason why w-p53 failed to prevent malignant transformation of esophageal epithelia.

关 键 词：食管癌 癌前上皮 P53基因 MRNA表达 

分 类 号：R735.1[医药卫生—肿瘤]