辣椒素对大鼠膀胱内压及CGRP-,NPY-免疫反应性神经的影响  

Influence of different concentration of intravesical capsaicin on intravesical pressure and calcitonin gene-related peptide-and neuropeptide Y-immunoreactive nerve fibers in urinary bladder of the rat

作　　者：林传友[1] AL-HOBISH Ahma Saleh 杨晓进[2] 周学锋[2] 邓云平[1] 茹立强[1] 

机构地区：[1]华中科技大学同济医学院神经生物学教研室,湖北武汉430030 [2]华中科技大学同济医学院同济医院小儿外科,湖北武汉430030

出　　处：《中国神经科学杂志》2001年第4期317-320,330,共5页

摘　　要：观察了不同浓度的辣椒素 (CAP)注入大鼠膀胱后 ,膀胱内压、膀胱容量的泌尿动力学变化及CAP对膀胱壁内含CGRP及NPY免疫反应性 (IR)神经的影响。结果发现 ,高浓度CAP(4mmol/L及 8mmol/L)处理后膀胱最大压力明显下降 ,而膀胱容量及残余尿量增加 (P 0 .0 1)。免疫组织化学发现 ,各种浓度的CAP均明显降低膀胱壁各层CGRP IR神经纤维的长度和密度 (P 0 .0 0 1)。结果表明 ,CAP能广泛耗竭膀胱组织内CAP敏感的一级传入神经末梢中的CGRP ,并表现出一定的浓度依赖性变化 ,此可能为降低膀胱内压 ,引起尿潴留和充溢性尿失禁的神经原性病理机制。这可能为临床上局部使用CAP治疗神经原性膀胱 (逼尿反射亢进 )。This paper studied the changes of intravesical pressure, bladder capacity and calcitonin gene related peptide (CGRP) and neuropeptide Y(NPY) immunoreactive(IR) nerve fibers in rat urinary bladder after intravesical instillations of different concentrations of capsaisin(CAP). The results showed that the maximal intravesical pressure decreased, while the bladder capacity and residual urine volume increased with the treatment of the higher (4 and 8 mmol/L) concentrations of CAP ( P <0.01). The different concentrations of CAP could significantly reduce both the length and the density of CGRP IR nerve fibers in the wall of the bladder ( P 0.001), suggesting that CAP could deplete the CGRP of capsaicin sensitive primary afferent fibers, and the changes were likely to be concentration dependent. This might be one of the neurogenic pathological mechanisms involved in the urinary retention and incontinence. Thus, the way of intravesical instillation of CAP could be useful in treatment of neurogenic bladder (detrusor huperreflexia) to relief frequency and urgency in passing urine.

关 键 词：辣椒素 膀胱 膀胱内压 CGRP NPY 大鼠 免疫反应性神经 泌尿动力学 

分 类 号：R965[医药卫生—药理学]