HLA-I分子对B7.1共刺激作用的影响  

Effect of HLA-I on the role of B7.1

作　　者：李增山[1] 隋延仿[1] 叶菁[1] 

机构地区：[1]第四军医大学病理教研室肿瘤抗原肽实验室,西安,710032

出　　处：《中国免疫学杂志》2001年第9期466-468,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金资助 (No.39770 82 7)

摘　　要：目的 :研究HLA I分子表达与B7 1分子的共刺激作用的相互关系 ,以探讨在肿瘤细胞所诱发的免疫排斥反应中共刺激分子对抗原递呈机制的影响。方法 :通过流式细胞仪 (FCM)检测转染共刺激分子B7 1编码基因前后其表面HLA I分子的表达情况。利用单克隆抗体阻断细胞表面HLA I分子 ,以外周血混合淋巴细胞对阻断前后的细胞行杀伤实验。结果 :表达B7 1的肝癌细胞其表面HLA I分子表达明显增高 (P <0 0 1) ,而γ INF刺激对细胞表面HLA I分子的表达情况并无影响(P >0 0 5 )。T淋巴细胞对转染后细胞的杀伤作用远远大于未转染组 (P <0 0 1) ,当表面HLA I分子被阻断后 ,T淋巴细胞的杀伤效应几乎降至零点。结论 :转染B7 1后肝癌细胞高表达HLA I分子 ,增强抗原提呈作用的同时又表达第二信号—共刺激分子B7 1,从而可诱发更强的免疫排斥反应。Objective:Study the relationship of HLA I and B7 1 to elucidate the role of costimulatory molecule on antigen presentation in immunological rejection.Methods:FCM was applied to detect the expression of B7 1 Then the cytotoxicity of PBML was observed before and after blocking the HLA I Results:The level of HLA I expression increased greatly(P<0 01)after transfection Also we found that the introduction of B7 1 into HHCC cells in vitro induced a strong immunity that was associated with T cells in contrast to the untransfected cells which B7 1 was not expressed (P<0 01) The cytotoxicity of T lymphocytes decreased nearly to zero level when HLA I were blocked Conclusion:The expression of B7 1 in HHCC conduce to high level of HLA I expression and increased effect of antigen presentation and costimulation in immunological reject reaction

关 键 词：肝癌细胞 B7.1 HLA-1 细胞毒 共刺激作用 

分 类 号：R735.7[医药卫生—肿瘤] R392.12[医药卫生—临床医学]