nm_(23-H1)与p21、p53蛋白在人卵巢癌中表达的相关性研究  

Expression and correlativity of nm_(23-H1) and p21, p53 proteins in patients with ovarian carcinoma

作　　者：郑美莲[1] 区宝祥[1] 李孟达[1] 陈丽珍[1] 李辉梅[1] 

机构地区：[1]中山医科大学肿瘤防治中心,广州510060

出　　处：《中国免疫学杂志》2001年第9期491-493,共3页Chinese Journal of Immunology

基　　金：中山医科大学肿瘤防治中心科研基金资助

摘　　要：目的 :研究人卵巢癌组织中肿瘤转移抑制基因nm2 3 H1 、抑癌基因p2 1 WAF1 及p53蛋白的表达 ,探讨它们在卵巢癌发生、发展中的作用。方法 :采用免疫印迹技术 ,对 74例卵巢癌组织及 2 1例卵巢非癌组织中nm2 3 H1 、p2 1 WAF1 及p53蛋白进行检测。结果 :在卵巢癌中nm2 3 H1 、p53蛋白的表达高于卵巢非癌组织 ,p2 1 WAF1 蛋白的表达呈下调 ;肿瘤发生转移时卵巢癌组织中nm2 3 H1 和p2 1WAF蛋白表达显著低于未发生肿瘤转移的癌组织。p53蛋白表达与肿瘤转移无关。nm2 3 H1 、p2 1 WAF1 蛋白与卵巢癌组织类型无关 ,但与卵巢癌的临床分期相关。p53蛋白的表达与卵巢癌组织类型及卵巢癌的临床分期无关。nm2 3 H1 与p53、p2 1 WAF1 蛋白间表达在卵巢癌中呈相关性。结论 :nm2 3 H1 、p53、p2 1 WAF1 基因在卵巢癌发生、发展中起一定的作用 ,nm2 3 H1 与p53、p2 1 WAF1 基因在卵巢癌发生、发展中可能起协同作用。Objective:To investigate expression of nm 23 H1 and p21 WAF1 , p53 in ovarian carcinoma Methods:Western blot was used for determination of nm 23 H1 , p21 WAF1 and p53 proteins expression in 74 ovarian carcinoma and 21 ovarian tissues without cancer Results:Expression of nm 23 H1 and p53 proteins were higher in cases with ovarian carcinoma than those in without cancer tissues p21 WAF1 protein expression was down regulated in ovarian carcinoma nm 23 H1 and p21 WAF1 proteins expression of tumor metastatic were significatly lower in patients with ovarian carcinoma than that in patient of not tumor metastatic Expression of p53 protein was not significantly correlate with tumor metastatic Expression of nm 23 H1 p53 and p21 WAF1 proteins were no correlation with histologic type However, p53 protein was correlated with histologic type Expression of nm 23 H1 and p21 WAF1 were correlated with clinical stage, but p53 was not In addition, there were correlation between nm 23 H1 protein and p53, p21 WAF1 proteins in ovarian carcinoma Conclusion:nm 23 H1 , p53 and p21 WAF1 genes may play a role in the formation and development of ovarian carcinoma They may act cooperatively in ovarian carcinoma

关 键 词：卵巢肿瘤 蛋白免疫印迹 NM23-H1 p21^WAFI p53 

分 类 号：R737.31[医药卫生—肿瘤]